概述

  • 产品名称Anti-Desmocollin 2抗体
    参阅全部 Desmocollin 2 一抗
  • 描述
    兔多克隆抗体to Desmocollin 2
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Dog, Human
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 480-676 of Human Desmocollin 2 isoform 2A and 2B (Uniprot ID = Q02487).

  • 阳性对照
    • 293T, A431, H1299, HeLa, MOLT4 and Raji lysates. OVCAR3 xenograft (IHC)

性能

应用

Our Abpromise guarantee covers the use of ab95967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 94 kDa.
IHC-P 1/100 - 1/500.

靶标

  • 功能Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. May contribute to epidermal cell positioning (stratification) by mediating differential adhesiveness between cells that express different isoforms.
  • 组织特异性Expressed in epithelia, myocardium and lymph nodes.
  • 疾病相关Defects in DSC2 are the cause of familial arrhythmogenic right ventricular dysplasia type 11 (ARVD11) [MIM:610476]; also known as arrhythmogenic right ventricular cardiomyopathy 11 (ARVC11). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall.
  • 序列相似性Contains 5 cadherin domains.
  • 结构域Calcium may be bound by the cadherin-like repeats.
  • 细胞定位Cell membrane. Cell junction > desmosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ARVD11 antibody
    • Cadherin family member 2 antibody
    • CDHF2 antibody
    • Desmocollin 3 antibody
    • Desmocollin-2 antibody
    • Desmocollin-3 antibody
    • Desmocollin2 antibody
    • Desmosomal glycoprotein II and III antibody
    • Desmosomal glycoprotein II antibody
    • Desmosomal glycoprotein II/III antibody
    • Desmosomal glycoprotein III antibody
    • DG2 antibody
    • DGII/III antibody
    • DKFZp686I11137 antibody
    • DSC 2 antibody
    • DSC2 antibody
    • DSC2_HUMAN antibody
    • DSC3 antibody
    see all

Anti-Desmocollin 2 antibody 图像

  • Anti-Desmocollin 2 antibody (ab95967) at 1/500 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 94 kDa
  • ab95967 at 1/500 dilution staining Desmocollin 2 in paraffin-embedded OVCAR3 xenograft by immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Anti-Desmocollin 2 antibody (ab95967)参考文献

This product has been referenced in:
  • Resnik N  et al. Desmosome assembly and cell-cell adhesion are membrane raft-dependent processes. J Biol Chem 286:1499-507 (2011). WB ; Dog . Read more (PubMed: 21071449) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (Epithelial Organoids)
Gel Running Conditions Reduced Denaturing (BioRad 4-15% TGX gel)
Loading amount 10 µg
Specification Epithelial Organoids
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mrs. Amanda Fairchild

Verified customer

提交于 Aug 03 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Dako serum free protein block as blocking agent for 30 minute(s) · Concentration: 0.25%
Antigen retrieval step Heat mediated
Sample Monkey Tissue sections (vagina)
Specification vagina
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 25 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"