重组Alexa Fluor® 488荧光Anti-Desmin抗体[Y66] - Cytoskeleton Marker (ab185033)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [Y66] to Desmin - Cytoskeleton Marker
- Suitable for: ICC/IF, Flow Cyt (Intra)
- Reacts with: Rat, Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
概述
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产品名称
Alexa Fluor® 488荧光Anti-Desmin抗体[Y66] - Cytoskeleton Marker
参阅全部 Desmin 一抗 -
描述
Alexa Fluor® 488荧光兔单克隆抗体[Y66] to Desmin - Cytoskeleton Marker -
宿主
Rabbit -
偶联物
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
经测试应用
适用于: ICC/IF, Flow Cyt (Intra)more details -
种属反应性
与反应: Rat, Human
预测可用于: Mouse, Guinea pig -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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表位
ab32362 reacts with an epitope located in the C terminal region of desmin. -
阳性对照
- ICC/IF: SV40LT-SMC cells. Flow Cyt (intra): SV40LT-SMC cells.
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
Y66 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Alexa Fluor® 647 Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab195177)
- HRP Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab195178)
- Biotin Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab197603)
- Alexa Fluor® 594 Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab203419)
- Alexa Fluor® 555 Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab203422)
- Anti-Desmin antibody [Y66] - Low endotoxin, Azide free (ab216616)
- APC Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab224934)
- PE Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab224935)
- Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab185033于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
1/50 - 1/100.
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Flow Cyt (Intra) |
1/50.
ab199091 - Rabbit monoclonal IgG (Alexa Fluor® 488), is suitable for use as an isotype control with this antibody. |
说明 |
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ICC/IF
1/50 - 1/100. |
Flow Cyt (Intra)
1/50. ab199091 - Rabbit monoclonal IgG (Alexa Fluor® 488), is suitable for use as an isotype control with this antibody. |
靶标
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功能
Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. -
疾病相关
Defects in DES are the cause of myopathy myofibrillar desmin-related (MFM-DES) [MIM:601419]; also known as desmin-related myopathy (DRM). A neuromuscular disorder characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, restrictive heart failure, and by myofibrillar destruction with intracytoplasmic accumulation of desmin-reactive deposits in cardiac and skeletal muscle cells.
Defects in DES are the cause of cardiomyopathy dilated type 1I (CMD1I) [MIM:604765]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in DES are the cause of neurogenic scapuloperoneal syndrome Kaeser type (Kaeser syndrome) [MIM:181400]. Kaeser syndrome is an autosomal dominant disorder with a peculiar scapuloperoneal distribution of weakness and atrophy. A large clinical variability is observed ranging from scapuloperoneal, limb grindle and distal phenotypes with variable cardiac or respiratory involvement. Facial weakness, dysphagia and gynaecomastia are frequent additional symptoms. Affected men seemingly bear a higher risk of sudden, cardiac death as compared to affected women. Histological and immunohistochemical examination of muscle biopsy specimens reveal a wide spectrum of findings ranging from near normal or unspecific pathology to typical, myofibrillar changes with accumulation of desmin. -
序列相似性
Belongs to the intermediate filament family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 1674 Human
- Entrez Gene: 13346 Mouse
- Entrez Gene: 64362 Rat
- Omim: 125660 Human
- SwissProt: P17661 Human
- SwissProt: P31001 Mouse
- SwissProt: P48675 Rat
- Unigene: 594952 Human
see all -
别名
- CMD1I antibody
- CSM1 antibody
- CSM2 antibody
see all
图片
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Overlay histogram showing SV40LT-SMC cells stained with ab185033 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab185033, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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ab185033 staining Desmin in SV40LT-SMC cells. The cells were fixed with 4% formaldehyde (10min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab185033 at 1/50 dilution overnight at +4°C (shown in green). AlexaFluor®350 WGA was used at a 1/200 dilution and incubated for 1h with the cells, to label plasma membranes (shown in blue). Nuclear DNA was labelled in red with 1.25 μM DRAQ5™ (ab108410).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab185033 被引用在 7 文献中.
- Chen L et al. The Apelin-Apelin Receptor Axis Triggers Cholangiocyte Proliferation and Liver Fibrosis During Mouse Models of Cholestasis. Hepatology 73:2411-2428 (2021). PubMed: 32964473
- Zhang D et al. Hepatoprotective effect of Xiayuxue decoction ethyl acetate fraction against carbon tetrachloride-induced liver fibrosis in mice via inducing apoptosis and suppressing activation of hepatic stellate cells. Pharm Biol 58:1229-1243 (2020). PubMed: 33332219
- Chen L et al. Pinealectomy or light exposure exacerbates biliary damage and liver fibrosis in cholestatic rats through decreased melatonin synthesis. Biochim Biophys Acta Mol Basis Dis 1865:1525-1539 (2019). PubMed: 30890428
- Wan Y et al. Knockout of a-calcitonin gene-related peptide attenuates cholestatic liver injury by differentially regulating cellular senescence of hepatic stellate cells and cholangiocytes. Lab Invest N/A:N/A (2019). PubMed: 30700848
- Zhou T et al. Knockdown of vimentin reduces mesenchymal phenotype of cholangiocytes in the Mdr2-/- mouse model of primary sclerosing cholangitis (PSC). EBioMedicine 48:130-142 (2019). PubMed: 31522982
- Zhu XJ et al. A Wnt/Notch/Pax7 signaling network supports tissue integrity in tongue development. J Biol Chem 292:9409-9419 (2017). PubMed: 28438836
- Wu N et al. Prolonged darkness reduces liver fibrosis in a mouse model of primary sclerosing cholangitis by miR-200b down-regulation. FASEB J 31:4305-4324 (2017). PubMed: 28634212