概述

  • 产品名称
  • 描述
    兔多克隆抗体to Desmin
  • 特异性
    In immunoblots, this antibody reacts with a 53 kD polypeptide. It does not react with vimentin or human cytokeratins. This antibody can be used to distinguish rhabdomyosarcomas and leiomyosarcomas from other soft tissue tumors, lymphomas, and carcinomas.
  • 经测试应用
    适用于: Electron Microscopy, IP, ELISA, WB, IHC-Frmore details
    不适用于: ICC/IF or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human, Syrian hamster
  • 免疫原

    Full length protein (Human).

  • 阳性对照
    • Heart muscle, smooth and skeletal muscle, leiomyoma and rhabdomyomas
  • 常规说明
    Prolonged fixation in buffered formalin can destroy the epitope.

性能

应用

Our Abpromise guarantee covers the use of ab8592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Electron Microscopy Use at an assay dependent concentration. PubMed: 17535849
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 53 kDa.
IHC-Fr Use at an assay dependent concentration.
  • 应用说明
    Is unsuitable for ICC/IF or IHC-P.
  • 靶标

    • 功能
      Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures.
    • 疾病相关
      Defects in DES are the cause of myopathy myofibrillar desmin-related (MFM-DES) [MIM:601419]; also known as desmin-related myopathy (DRM). A neuromuscular disorder characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, restrictive heart failure, and by myofibrillar destruction with intracytoplasmic accumulation of desmin-reactive deposits in cardiac and skeletal muscle cells.
      Defects in DES are the cause of cardiomyopathy dilated type 1I (CMD1I) [MIM:604765]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
      Defects in DES are the cause of neurogenic scapuloperoneal syndrome Kaeser type (Kaeser syndrome) [MIM:181400]. Kaeser syndrome is an autosomal dominant disorder with a peculiar scapuloperoneal distribution of weakness and atrophy. A large clinical variability is observed ranging from scapuloperoneal, limb grindle and distal phenotypes with variable cardiac or respiratory involvement. Facial weakness, dysphagia and gynaecomastia are frequent additional symptoms. Affected men seemingly bear a higher risk of sudden, cardiac death as compared to affected women. Histological and immunohistochemical examination of muscle biopsy specimens reveal a wide spectrum of findings ranging from near normal or unspecific pathology to typical, myofibrillar changes with accumulation of desmin.
    • 序列相似性
      Belongs to the intermediate filament family.
    • 细胞定位
      Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CMD1I antibody
      • CSM1 antibody
      • CSM2 antibody
      • DES antibody
      • DESM_HUMAN antibody
      • Desmin antibody
      • FLJ12025 antibody
      • FLJ39719 antibody
      • FLJ41013 antibody
      • FLJ41793 antibody
      • Intermediate filament protein antibody
      • OTTHUMP00000064865 antibody
      see all

    Anti-Desmin antibody 图像

    • ab8592 was used to stain mouse prostate.

    • All lanes : Anti-Desmin antibody (ab8592) at 1/1000 dilution

      Lane 1 : RMS13 cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : Human skeletal muscle tissue lysate
      Lane 4 : Human heart tissue lysate
      Lane 5 : C2C12 cell lysate
      Lane 6 : Mouse skeletal muscle tissue lysate
      Lane 7 : Mouse heart tissue lysate
      Lane 8 : L6 cell lysate
      Lane 9 : Rat heart tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Observed band size : 53 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

      Blocking and dilution buffer: 5% NFDM/TBST.

    Anti-Desmin antibody (ab8592)参考文献

    This product has been referenced in:
    • Echigoya Y  et al. Effects of systemic multiexon skipping with peptide-conjugated morpholinos in the heart of a dog model of Duchenne muscular dystrophy. Proc Natl Acad Sci U S A 114:4213-4218 (2017). WB ; Dog . Read more (PubMed: 28373570) »
    • Sansone P  et al. Evolution of Cancer Stem-like Cells in Endocrine-Resistant Metastatic Breast Cancers Is Mediated by Stromal Microvesicles. Cancer Res 77:1927-1941 (2017). Read more (PubMed: 28202520) »

    See all 57 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Heart)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    20 µg
    Specification
    Heart
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Aug 21 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue (Embryonic Mouse Heart Section)
    Permeabilization
    Yes - Tween-20
    Specification
    Embryonic Mouse Heart Section
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Jul 25 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
    Sample
    Mouse Cell (Pericytes (xenograft tumour tissue))
    Specification
    Pericytes (xenograft tumour tissue)
    Permeabilization
    No
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 May 10 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Colon)
    Specification
    Colon
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - triton 0,1% in PBS
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 24°C
    Username

    Dr. Alessandro Gandelli

    Verified customer

    提交于 Mar 01 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Intestine)
    Specification
    Intestine
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    提交于 Aug 28 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Colon)
    Specification
    Colon
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - .1% triton x-100
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    提交于 Apr 19 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (hek293)
    Loading amount
    50 µg
    Specification
    hek293
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    提交于 Oct 26 2010

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (brain pericytes)
    Specification
    brain pericytes
    Username

    Abcam user community

    Verified customer

    提交于 Oct 15 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (muscle)
    Loading amount
    50 µg
    Specification
    muscle
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Aug 27 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (dermal fibroblasts)
    Specification
    dermal fibroblasts
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton-X-100
    Blocking step
    fetal calf serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 Feb 25 2010

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