概述

  • 产品名称Anti-DEP1抗体
    参阅全部 DEP1 一抗
  • 描述
    兔多克隆抗体to DEP1
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from residues 1200 - 1300 of Human DEP1.

  • 阳性对照
    • This antibody gave a positive signal in recombinant DEP1 protein

性能

应用

Our Abpromise guarantee covers the use of ab10639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
WB
  • 应用说明IHC-P: 1/50 - 1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. Enzymatic antigen retrieval has also been attempted, however this was not as successful at the microwave method.

    WB: 1/500. Detects a band of approximately ~60 kDa. Can be blocked with DEP1 protein (phosphatase domain) (ab13229).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Tyrosine phosphatase which dephosphorylates or contributes to the dephosphorylation of CTNND1, PDGFRB, MET, RET (variant MEN2A), KDR, LYN, SRC, MAPK1, MAPK3, EGFR, TJP1, OCLN, PIK3R1 and PIK3R2. Plays a role in cell adhesion, migration, proliferation and differentiation. Involved in vascular development. Regulator of macrophage adhesion and spreading. Positively affects cell-matrix adhesion. Positive regulator of platelet activation and thrombosis. Negative regulator of cell proliferation. Negative regulator of PDGF-stimulated cell migration; through dephosphorylation of PDGFR. Positive regulator of endothelial cell survival, as well as of VEGF-induced SRC and AKT activation; through KDR dephosphorylation. Negative regulator of EGFR signaling pathway; through EGFR dephosphorylation. Enhances the barrier function of epithelial junctions during reassembly. Negatively regulates T-cell receptor (TCR) signaling. Upon T-cell TCR activation, it is up-regulated and excluded from the immunological synapses, while upon T-cell-antigen presenting cells (APC) disengagement, it is no longer excluded and can dephosphorylate PLCG1 and LAT to down-regulate prolongation of signaling.
    • 组织特异性Expressed in the promyelocytic cell line HL60, the granulocyte-macrophage colony-stimulating factor-dependent leukemic cell line F-36P, and the interleukin-3 and erythropoietin-dependent leukemic cell line F-36E. Expressed predominantly in epithelial cells and lymphocytes. Enhanced expression at high cell density.
    • 序列相似性Belongs to the protein-tyrosine phosphatase family. Receptor class 3 subfamily.
      Contains 9 fibronectin type-III domains.
      Contains 1 tyrosine-protein phosphatase domain.
    • 翻译后修饰N- and O-glycosylated.
    • 细胞定位Cell membrane. Cell projection > ruffle membrane. Cell junction. After T cell stimulation, it is temporarily excluded from immunological synapses.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CD 148 antibody
      • CD148 antibody
      • CD148 antigen antibody
      • Density enhanced phosphatase 1 antibody
      • Density-enhanced phosphatase 1 antibody
      • DEP 1 antibody
      • DEP-1 antibody
      • HPTP eta antibody
      • HPTPeta antibody
      • Human density enhanced phosphatase 1 antibody
      • Protein tyrosine phosphatase eta antibody
      • Protein tyrosine phosphatase receptor type J antibody
      • Protein tyrosine phosphatase receptor type J polypeptide antibody
      • Protein-tyrosine phosphatase eta antibody
      • Protein-tyrosine phosphatase receptor type J antibody
      • PTPJ antibody
      • Ptprj antibody
      • PTPRJ_HUMAN antibody
      • R PTP Eta antibody
      • R-PTP-eta antibody
      • R-PTP-J antibody
      • Receptor type tyrosine protein phosphatase eta antibody
      • Receptor-type tyrosine-protein phosphatase eta antibody
      • SCC 1 antibody
      • SCC1 antibody
      • susceptibility to colon cancer 1, mouse, homolog of antibody
      see all

    Anti-DEP1 antibody 图像

    • Recombinant PTP phosphatase domains (5µg per lane) were probed with ab10639 at 1/500.  The antibody recognises the recombinant DEP1(PTPRJ) fusion protein at ~60 kDa.

      Lane 1: DEP1 (PTPRJ)
      Lane 2: GLEPP1 (PTPRO)
      Lane 3: PTP beta (PTPRB)
      Lane 4: PTP sigma (PTPRS)
      Lane 5: PCPTP1 (PTPRR)

    • Liver carcinoma tissue was fixed in 10% formalin at 4°C overnight and paraffin embedded. Following blocking the tissue sections were incubated with ab10639 (1/100 dilution) at 37°C for 1 hour, and with the secondary antibody at 37°C for 30 minutes. The slides were counterstained with Hematoxylin. Washes were carried out using TBS. Heat mediated antigen retrieval was carried out using the Microwave method before commencing the staining protocol.

      ab10639 is clearly localised to the cytoplasm of the tissue (brown). Nuclear staining can be seen in blue. 100x magnification.



    Anti-DEP1 antibody (ab10639)参考文献

    ab10639 has not yet been referenced specifically in any publications.

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