概述

  • 产品名称Anti-DENND1B抗体
    参阅全部 DENND1B 一抗
  • 描述
    兔多克隆抗体to DENND1B
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Zebrafish
  • 免疫原

    Synthetic peptide: PVNLSVNQEI FIACEQVLKD QPALVPHSYF IAPDVTGLPT IPESRNLTEY , corresponding to a region within internal sequence amino acids 144 - 193 of Human DENND1B isoform 1 (NP_659414)

  • 阳性对照
    • 293T cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab84347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Guanine nucleotide exchange factor (GEF) for RAB35. May function as a clathrin-associated sorting protein for RAB35, whereby during clathrin-mediated endocytosis, binds the AP-2 complex and inactived RAB35, then activates RAB35 for its subsequent function at endosomes.
  • 组织特异性Highly expressed in dendritic and natural killer cells and at lower levels in other myeloid lineage cells and in pituitary. Significantly up-regulated in effector memory T cells as compared with naive T cells.
  • 序列相似性Contains 1 dDENN domain.
    Contains 1 DENN domain.
    Contains 1 uDENN domain.
  • 翻译后修饰Isoform 5 is phosphorylated on Tyr-475, Tyr-484, Ser-514 and Ser-652.
  • 细胞定位Cytoplasm > cytosol. Cytoplasmic vesicle > clathrin-coated vesicle.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C1ORF18 antibody
    • C1orf218 antibody
    • Chromosome 1 open reading frame 218 antibody
    • Connecdenn 2 antibody
    • DEN1B_HUMAN antibody
    • DENN domain containing protein 1B antibody
    • DENN domain-containing protein 1B antibody
    • DENN MADD domain containing 1B antibody
    • DENND1B antibody
    • DKFZp547O0715 antibody
    • FAM31B antibody
    • Family with sequence similarity 31 member B antibody
    • FLJ20054 antibody
    • FLJ21179 antibody
    • FLJ33888 antibody
    • MGC103810 antibody
    • MGC27044 antibody
    • Protein FAM31B antibody
    • RP11 53I24.2 antibody
    see all

Anti-DENND1B antibody 图像

  • Anti-DENND1B antibody (ab84347) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 48 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)

Anti-DENND1B antibody (ab84347)参考文献

ab84347 has not yet been referenced specifically in any publications.

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