Anti-Dengue Virus NS1 glycoprotein抗体[DN3] (ab41616)

概述

  • 产品名称
    Anti-Dengue Virus NS1 glycoprotein抗体[DN3]
    参阅全部 Dengue Virus NS1 glycoprotein 一抗
  • 描述
    小鼠单克隆抗体[DN3] to Dengue Virus NS1 glycoprotein
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4
  • 免疫原

    Full length native protein purified from Dengue Virus 2 (16681) infected supernatant.

  • 阳性对照
    • It is important to ensure that DEN4 infection is effective. DEN4 is notoriously difficult to grow to good titres and NS1 antigens will not be found if infection is not well established.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 10mM Sodium Azide
  • 纯度
    Tissue culture supernatant
  • 克隆
    单克隆
  • 克隆编号
    DN3
  • 同种型
    IgG1
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab41616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50 - 1/100. Use under non reducing condition. Detects a band of approximately 40, 80 kDa (predicted molecular weight: 40, 80 kDa). Block membrane with PBS containing 5% non-fat skimmed milk (PBS-SM) for 30 minutes. Wash membrane and probe with serum diluted in PBS-SM with 10 mM sodium azide by incubating overnight on a rocker at room temperature.
ICC/IF 1/5 - 1/20.

靶标

  • 相关性
    NS1 is one of 7 Dengue Virus non-structural proteins which are thought to be involved in viral replication. NS1 exists as a monomer in its immature form but is rapidly processed in the endoplasmic reticulum to form a stable dimer. A small amount of NS1 remains associated with intracellular organelles where it is thought to be involved in viral replication. The rest of NS1 is found either associated with the plasma membrane or secreted as a soluble hexadimer. NS1 is essential for viral viability but its precise biological function is unknown. Antibodies raised in response to NS1 in viral infection can cross react with cell surface antigens on epithelial cells and platelets and this has been implicated in the development of Dengue Hemorrhagic fever.
  • 数据库链接
    • 别名
      • Dengue NS1 antibody
      • Dengue Virus non structural protein 1 antibody

    Anti-Dengue Virus NS1 glycoprotein antibody [DN3] 图像

    • All lanes : Anti-Dengue Virus NS1 glycoprotein antibody [DN3] (ab41616)

      Lane 1 : Dengue Virus 1 infected C6/36 cell lysate (unheated)
      Lane 2 : Dengue Virus 1 infected C6/36 cell lysate (boiled)
      Lane 3 : Dengue Virus 2 (NGC) infected C6/36 cell lysate (unheated)
      Lane 4 : Dengue Virus 2 (NGC) infected C6/36 cell lysate (boiled)
      Lane 5 : Dengue Virus 2 (16681) infected C6/36 cell lysate (unheated)
      Lane 6 : Dengue Virus 2 (16681) infected C6/36 cell lysate (boiled)
      Lane 7 : Dengue Virus 3 infected C6/36 cell lysate (unheated)
      Lane 8 : Dengue Virus 3 infected C6/36 cell lysate (boiled)
      Lane 9 : Dengue Virus 4 infected C6/36 cell lysate (unheated)
      Lane 10 : Dengue Virus 4 infected C6/36 cell lysate (boiled)


      Predicted band size : 40, 80 kDa
      Observed band size : 40,80 kDa
    • Dengue Virus 2 infected Vero cells stained according to protocol with ab41349 antibody against Dengue Virus envelope glycoprotein NS1 (green). Cell nuclei are blue.
    • ab41616 staining NS1 glycoprotein in Dengue Virus 2 infected Huh-7.5 cells by Immunocytochemistry/ Immunofluorescence.
      Huh-7.5 cells were plated on glass coverslips, infected with Dengue Virus 2, fixed in methanol, and blocked, and antibody was added in 1× PBS + 0.1% saponin. Antibodies used include FASN polyclonal, left image (ab3844) or NS1 monoclonal antibody, middle image (ab41616). Secondary antibodies were species-appropriate Alexa-Fluor 488 or 594 secondary antibody. Stained coverslips were mounted with DAPI, right merged image.

    Anti-Dengue Virus NS1 glycoprotein antibody [DN3] (ab41616)参考文献

    This product has been referenced in:
    • Cai H  et al. Cell-death-inducing DFFA-like Effector B Contributes to the Assembly of Hepatitis C Virus (HCV) Particles and Interacts with HCV NS5A. Sci Rep 6:27778 (2016). Read more (PubMed: 27282740) »
    • Simon AY  et al. Dengue virus binding and replication by platelets. Blood 126:378-85 (2015). Read more (PubMed: 25943787) »

    See all 7 Publications for this product

    Product Wall

    Application
    Flow Cytometry
    Fixation
    BD CytoFix/CytoPerm
    Permeabilization
    Yes - BD CytoFix/CytoPerm
    Sample
    Human Cell (HeLa at 62hpi with DV2)
    Specification
    HeLa at 62hpi with DV2
    Gating Strategy
    single cells by FSC and SSC
    Preparation
    Cell harvesting/tissue preparation method: spatula scraping
    Sample buffer: cold PBS
    Username

    Lee Gehrke

    Verified customer

    提交于 Oct 07 2013

    Thank you for contacting us.

    DN3 is just a clone name. The antibody is reactive to all serotypes.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Thank you for your message which has been forwarded to me as my colleague Anja is currently out of the office.

    We appreciate the feedback from the customer, thank you for sending this information.

    In order to obtain the discount, ...

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    ELISA
    Sample
    Escherichia coli Recombinant protein (Dengue NS1)
    Specification
    Dengue NS1
    Blocking step
    BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Type
    Indirect
    Username

    Abcam user community

    Verified customer

    提交于 Jul 26 2012

    Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable...

    Read More

    Thank youand your customer very much for your interest in anti dengue virusNS1 antibodies.

    I can confirm that we have three in our catalog: ab41490, ab41616 and ab41623.

    To our knowledge, none of these antibodies has been tested in...

    Read More

    Thank youfor your inquiry.

    I can confirm that we do have three anti NS1 antibodies for Dengue virus in our catalog..

    Isotype control are not included and have to be purchased separately.

    ab41616 (Anti-Dengue Virus NS1 glyc...

    Read More

    Gracias por tu respuesta.
    He consultado esta información con mis compañeros del laboratorio.
    La glicoproteína (ab64456) contiene toda la secuencia excepto el dominio transmembranal. Además contiene 2x6 His tags, uno localizado en el extremo N...

    Read More

    Thank you for your inquiry.

    ab41616 is a mouse monoclonal IgG1 with a kappa light chain, un-conjugated.

    Therefore a isotype control is needed that is also un-conjugated and of the IgG1 Isotype (preferably with a kappa light chain). ...

    Read More

    Thank you for contacting us.
    I am sorry to hear you are having problems with one of our products. I appreciate the details provided. I am afraid that product ab41616 is not guaranteed to work in ELISA. We have tested this antibody in WB and ICC, b...

    Read More

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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