Anti-Dengue Virus NS1 glycoprotein抗体[DN2] (ab41623)

Abreviews (2)Q&A (6)Specific References (5)

概述

  • 产品名称
    Anti-Dengue Virus NS1 glycoprotein抗体[DN2]
    参阅全部 Dengue Virus NS1 glycoprotein 一抗
  • 描述
    小鼠单克隆抗体[DN2] to Dengue Virus NS1 glycoprotein
  • 宿主
    Mouse
  • 特异性
    Recognises NS1 from both Dengue Virus 2 strains, 16681 and NGC.
  • 经测试应用
    适用于: Flow Cyt, WB, ICC/IFmore details
  • 种属反应性
    与反应: Dengue virus 2
  • 免疫原

    Full length native protein purified from Dengue Virus 2 (16681) infected supernatant

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 10mM Sodium Azide
  • 纯度
    Tissue culture supernatant
  • 克隆
    单克隆
  • 克隆编号
    DN2
  • 同种型
    IgG1
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab41623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/80.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
WB 1/50 - 1/100. Use under non reducing condition. Predicted molecular weight: 40 kDa.
ICC/IF 1/5 - 1/20.

靶标

  • 相关性
    NS1 is one of 7 Dengue Virus non-structural proteins which are thought to be involved in viral replication. NS1 exists as a monomer in its immature form but is rapidly processed in the endoplasmic reticulum to form a stable dimer. A small amount of NS1 remains associated with intracellular organelles where it is thought to be involved in viral replication. The rest of NS1 is found either associated with the plasma membrane or secreted as a soluble hexadimer. NS1 is essential for viral viability but its precise biological function is unknown. Antibodies raised in response to NS1 in viral infection can cross react with cell surface antigens on epithelial cells and platelets and this has been implicated in the development of Dengue Hemorrhagic fever.
  • 数据库链接
    • 别名
      • Dengue NS1 antibody
      • Dengue Virus non structural protein 1 antibody

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-Dengue Virus NS1 glycoprotein antibody [DN2] (ab41623)
      Immunocytochemistry/ Immunofluorescence - Anti-Dengue Virus NS1 glycoprotein antibody [DN2] (ab41623)This image is courtesy of an Abreview submitted by Lee Gehrke

      ab41623 staining Dengue Virus NS1 glycoprotein (green) in Human BHK cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed and permeabilized with CytoFix/CytoPerm and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/20) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

      See Abreview

    • Flow Cytometry - Anti-Dengue Virus NS1 glycoprotein antibody [DN2] (ab41623)
      Flow Cytometry - Anti-Dengue Virus NS1 glycoprotein antibody [DN2] (ab41623)This image is courtesy of an Abreview submitted by Lee Gehrke

      ab41623 staining Dengue Virus NS1 glycoprotein in HeLa cells at 62hpi with DV2 by Flow Cytometry. Cells were fixed and permeabilized with BD CytoFix/CytoPerm. The sample was incubated with the primary antibody (1/80 in CytoFix/CytoPerm) for 1 hour at 37°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG (1/150) was used as the secondary antibody.
      Gating Strategy: Single cells by FSC and SSC.

       

      See Abreview

    • Western blot - Anti-Dengue Virus NS1 glycoprotein antibody [DN2] (ab41623)
      Western blot - Anti-Dengue Virus NS1 glycoprotein antibody [DN2] (ab41623)
      All lanes : Anti-Dengue Virus NS1 glycoprotein antibody [DN2] (ab41623)

      Lane 1 : Dengue Virus 2 (NGC)infected C6/36 cell lysate (unheated)
      Lane 2 : Dengue Virus 2 (NGC)infected C6/36 cell lysate (boiled)
      Lane 3 : Dengue Virus 2 (16681)infected C6/36 cell lysate (unheated)
      Lane 4 : Dengue Virus 2 (16681)infected C6/36 cell lysate (boiled)

      Predicted band size: 40 kDa
      Observed band size: 40,80 kDa (why is the actual band size different from the predicted?)



      NS1 exists as a dimer (~80 kDa) in unheated samples but is dissociated into a monomer (~40 kDa) when samples are boiled. Ab41623 recognised both forms of NS1.

    文献

    This product has been referenced in:
    • Durbin AF  et al. RNAs Containing Modified Nucleotides Fail To Trigger RIG-I Conformational Changes for Innate Immune Signaling. MBio 7:N/A (2016). Read more (PubMed: 27651356) »
    • Scaturro P  et al. Dengue Virus Non-structural Protein 1 Modulates Infectious Particle Production via Interaction with the Structural Proteins. PLoS Pathog 11:e1005277 (2015). Read more (PubMed: 26562291) »

    See all 5 Publications for this product

    发表研究结果有使用 ab41623？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

    提交评价 提交问题

    8 reviews or Q&A

    10
    Votes: Highest First

    I'm trying to detect the dengue NS1 inside human blood plasma samples using microfluidic platform. I'm not sure which serotype of dengue NS1 that I have. I'm looking for anti-dengue NS1 antibody from Abcam so that I can detect those NS1 in plasma sampl...

    Read More

    I recommend ab138696, which has been validated in ELISA and shown to cross react with serotypes 1, 2, 3, and 4. A link to this antibody is below:



    http://www.abcam.com/dengue-virus-ns1-glycoprotein-antibody-b1424m-ab138696.html

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Dengue Virus NS1 glycoprotein antibody [DN2]

     Excellent
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Sample
    Human Cell (BHK)
    Specification
    BHK
    Permeabilization
    Yes - BD CytoFix/CytoPerm
    Fixative
    BD CytoFix/CytoPerm
    Read More
    abreview image
    Username

    Lee Gehrke

    Verified customer

    提交于 Oct 11 2013

    Flow Cytometry abreview for Anti-Dengue Virus NS1 glycoprotein antibody [DN2]

     Excellent
    Abreviews
    Application
    Flow Cytometry
    Fixation
    BD CytoFix/CytoPerm
    Permeabilization
    Yes - BD CytoFix/CytoPerm
    Sample
    Human Cell (HeLa at 62hpi with DV2)
    Specification
    HeLa at 62hpi with DV2
    Gating Strategy
    single cells by FSC and SSC
    Preparation
    Cell harvesting/tissue preparation method: spatula scraping
    Sample buffer: cold PBS
    Read More
    abreview image
    Username

    Lee Gehrke

    Verified customer

    提交于 Oct 07 2013

    Dear Technical support teams,

    Would you pls suggest me aboutantibody for dengue
    > > non-structural protein 1 (NS1) , that be able to use in ELISA method.

    The customer found only the anti-dengue NS1 for ICF or WB applica...

    Read More

    Thank youand your customer very much for your interest in anti dengue virusNS1 antibodies.

    I can confirm that we have three in our catalog: ab41490, ab41616 and ab41623.

    To our knowledge, none of these antibodies has been tested in...

    Read More

    Thanks for the mail information, Please find that we need below kits.


    Dengue NS1 Antibodies.

    Isotype Controls Kit.


    Please inform its literature prices and expiry. Are you accept payment by credit card?
    ...

    Read More

    Thank youfor your inquiry.

    I can confirm that we do have three anti NS1 antibodies for Dengue virus in our catalog..

    Isotype control are not included and have to be purchased separately.

    ab41616 (Anti-Dengue Virus NS1 glyc...

    Read More

    Thank you for your reply. I realized that into the datasheet of ab41490, ab41616 and ab41623, I couldn't find about the specific storage buffer. The only information is 10mM Sodium Azide. I would like to know about the storage buffer because the con...

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    Thank you for your reply. These three antibodies are provided as tissue culture supernatant. This is stated in the purity section of the datasheet. It has some sodium azide added to it, but is not provided in a storage buffer. I am sorry we do not know...

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    Hi, I've recently purchased the following products: ab41490, ab41623 and ab41616, which are anti-ns1 (DENV) mouse monoclonal from DN1, DN2 and DN3 clones, respectively. I would like to know if these antibodies recognize conformational epitopes of DE...

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    Thank you for your enquiry. Wecan only confirm that ab41490 DN1 antibody will recognize NS1 protein from an MVA NS1 recombinant.I am sorry, we have no data from NS1 expressed in E coli or P pastoris However, if your recombinant proteins (ie NS1 from DE...

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    LOT NUMBER *****ORDER NUMBER ***** DESCRIPTION OF THE PROBLEM Target protein is not precipitated SAMPLE no PRIMARY ANTIBODY Dear Sir, I received the mouse monoclonal DN2 to dengue virus NS1 glycoprotein antibody today. Thanks for the prompt service. Ho...

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    Thank you for contacting Abcam. As ab41623 is a tissue culture supernatant, the concentration has not been determined. The following dilutions have been recommended, for western blot 1/50-1-100 and for ICC/IF, 1/5-1/20. The antibody has not been tested...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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