Anti-Dengue Virus NS1 glycoprotein抗体[DN2] (ab41623)

概述

  • 产品名称Anti-Dengue Virus NS1 glycoprotein抗体[DN2]
    参阅全部 Dengue Virus NS1 glycoprotein 一抗
  • 描述
    小鼠单克隆抗体[DN2] to Dengue Virus NS1 glycoprotein
  • 特异性Recognises NS1 from both Dengue Virus 2 strains, 16681 and NGC.
  • 经测试应用适用于: Flow Cyt, WB, ICC/IFmore details
  • 种属反应性
    与反应: Dengue Virus 2
  • 免疫原

    Full length native protein purified from Dengue Virus 2 (16681) infected supernatant

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 10mM Sodium Azide
  • 纯度Tissue culture supernatant
  • 克隆单克隆
  • 克隆编号DN2
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab41623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/80. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB 1/50 - 1/100. Use under non reducing condition. Predicted molecular weight: 40 kDa.
ICC/IF 1/5 - 1/20.

靶标

  • 相关性NS1 is one of 7 Dengue Virus non-structural proteins which are thought to be involved in viral replication. NS1 exists as a monomer in its immature form but is rapidly processed in the endoplasmic reticulum to form a stable dimer. A small amount of NS1 remains associated with intracellular organelles where it is thought to be involved in viral replication. The rest of NS1 is found either associated with the plasma membrane or secreted as a soluble hexadimer. NS1 is essential for viral viability but its precise biological function is unknown. Antibodies raised in response to NS1 in viral infection can cross react with cell surface antigens on epithelial cells and platelets and this has been implicated in the development of Dengue Hemorrhagic fever.
  • 数据库链接
    • 别名
      • Dengue NS1 antibody
      • Dengue Virus non structural protein 1 antibody

    Anti-Dengue Virus NS1 glycoprotein antibody [DN2] 图像

    • ab41623 staining Dengue Virus NS1 glycoprotein (green) in Human BHK cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed and permeabilized with CytoFix/CytoPerm and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/20) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

      See Abreview

    • ab41623 staining Dengue Virus NS1 glycoprotein in HeLa cells at 62hpi with DV2 by Flow Cytometry. Cells were fixed and permeabilized with BD CytoFix/CytoPerm. The sample was incubated with the primary antibody (1/80 in CytoFix/CytoPerm) for 1 hour at 37°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG (1/150) was used as the secondary antibody.
      Gating Strategy: Single cells by FSC and SSC.

       

      See Abreview

    • All lanes : Anti-Dengue Virus NS1 glycoprotein antibody [DN2] (ab41623)

      Lane 1 : Dengue Virus 2 (NGC)infected C6/36 cell lysate (unheated)
      Lane 2 : Dengue Virus 2 (NGC)infected C6/36 cell lysate (boiled)
      Lane 3 : Dengue Virus 2 (16681)infected C6/36 cell lysate (unheated)
      Lane 4 : Dengue Virus 2 (16681)infected C6/36 cell lysate (boiled)


      Predicted band size : 40 kDa
      Observed band size : 40,80 kDa (why is the actual band size different from the predicted?)
      NS1 exists as a dimer (~80 kDa) in unheated samples but is dissociated into a monomer (~40 kDa) when samples are boiled. Ab41623 recognised both forms of NS1.

    Anti-Dengue Virus NS1 glycoprotein antibody [DN2] (ab41623)参考文献

    This product has been referenced in:
    • Scaturro P  et al. Dengue Virus Non-structural Protein 1 Modulates Infectious Particle Production via Interaction with the Structural Proteins. PLoS Pathog 11:e1005277 (2015). Read more (PubMed: 26562291) »
    • Immenschuh S  et al. Antibodies against dengue virus nonstructural protein-1 induce heme oxygenase-1 via a redox-dependent pathway in Human endothelial cells. Free Radic Biol Med : (2012). Read more (PubMed: 23103292) »

    See all 3 Publications for this product

    Product Wall

    I recommend ab138696, which has been validated in ELISA and shown to cross react with serotypes 1, 2, 3, and 4. A link to this antibody is below:



    http://www.abcam.com/dengue-virus-ns1-glycoprotein-antibody-b1424m-ab138696.html

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Sample Human Cell (BHK)
    Specification BHK
    Permeabilization Yes - BD CytoFix/CytoPerm
    Fixative BD CytoFix/CytoPerm
    Username

    Lee Gehrke

    Verified customer

    提交于 Oct 11 2013

    Application Flow Cytometry
    Fixation BD CytoFix/CytoPerm
    Permeabilization Yes - BD CytoFix/CytoPerm
    Sample Human Cell (HeLa at 62hpi with DV2)
    Specification HeLa at 62hpi with DV2
    Gating Strategy single cells by FSC and SSC
    Preparation Cell harvesting/tissue preparation method: spatula scraping
    Sample buffer: cold PBS
    Username

    Lee Gehrke

    Verified customer

    提交于 Oct 07 2013

    Thank youand your customer very much for your interest in anti dengue virusNS1 antibodies.

    I can confirm that we have three in our catalog: ab41490, ab41616 and ab41623.

    To our knowledge, none of these antibodies has been tested in...

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    Thank youfor your inquiry.

    I can confirm that we do have three anti NS1 antibodies for Dengue virus in our catalog..

    Isotype control are not included and have to be purchased separately.

    ab41616 (Anti-Dengue Virus NS1 glyc...

    Read More

    Thank you for your reply. These three antibodies are provided as tissue culture supernatant. This is stated in the purity section of the datasheet. It has some sodium azide added to it, but is not provided in a storage buffer. I am sorry we do not know...

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    Thank you for your enquiry. Wecan only confirm that ab41490 DN1 antibody will recognize NS1 protein from an MVA NS1 recombinant.I am sorry, we have no data from NS1 expressed in E coli or P pastoris However, if your recombinant proteins (ie NS1 from DE...

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    Thank you for contacting Abcam. As ab41623 is a tissue culture supernatant, the concentration has not been determined. The following dilutions have been recommended, for western blot 1/50-1-100 and for ICC/IF, 1/5-1/20. The antibody has not been tested...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"