Anti-Dengue Virus E glycoprotein抗体[DE1] (ab41349)

概述

  • 产品名称Anti-Dengue Virus E glycoprotein抗体[DE1]
    参阅全部 Dengue Virus E glycoprotein 一抗
  • 描述
    小鼠单克隆抗体[DE1] to Dengue Virus E glycoprotein
  • 特异性This antibody reacts with a conformational epitope on the envelope gylcoprotein of Dengue Virus 1, 2, 3 and 4.
  • 经测试应用适用于: ICC/IF, ELISA, Electron Microscopy, Dot blot, Flow Cytmore details
    不适用于: WB
  • 种属反应性
    与反应: Dengue Virus 1, Dengue Virus 2, Dengue Virus 3, Dengue Virus 4
  • 免疫原

    Full length native protein purified from Dengue Virus 2 (16681) infected supernatant

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 10mM Sodium Azide
  • 纯度Tissue culture supernatant
  • 克隆单克隆
  • 克隆编号DE1
  • 同种型IgG2b
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab41349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/5 - 1/20.
ELISA Use at an assay dependent concentration. This antibody has been used in ELISA for the detection of degue IgM in patient serum samples.
Electron Microscopy Use at an assay dependent concentration. PubMed: 24681578
Dot blot Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. PubMed: 24625456ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for WB.
  • 靶标

    • 相关性Dengue virus E glycoprotein is thought to be essential for membrane fusion and is also involved in binding to cellular receptors. It is the primary antigen responsible for inducing the protective immune response.
    • 数据库链接
      • 别名
        • Dengue E glycoprotein antibody
        • Dengue envelope glycoprotein antibody
        • Dengue glycoprotein E antibody
        • Dengue virus envelope glycoprotein antibody
        • Dengue virus glycoprotein E antibody
        see all

      Anti-Dengue Virus E glycoprotein antibody [DE1] 图像

      • Dengue Virus 2 infected Vero cells stained with ab41349 antibody against Dengue Virus envelope glycoprotein E (green). Cell nuclei are blue.
      • Dengue Virus 2 infected Vero cells stained according to protocol with ab41349 antibody against Dengue Virus envelope glycoprotein E (green). Cell nuclei are blue.

      Anti-Dengue Virus E glycoprotein antibody [DE1] (ab41349)参考文献

      This product has been referenced in:
      • Al-Alimi AA  et al. Dengue virus type 2 (DENV2)-induced oxidative responses in monocytes from glucose-6-phosphate dehydrogenase (G6PD)-deficient and G6PD normal subjects. PLoS Negl Trop Dis 8:e2711 (2014). Flow Cyt . Read more (PubMed: 24625456) »
      • Burlaud-Gaillard J  et al. Correlative scanning-transmission electron microscopy reveals that a chimeric flavivirus is released as individual particles in secretory vesicles. PLoS One 9:e93573 (2014). Electron Microscopy . Read more (PubMed: 24681578) »

      See all 4 Publications for this product

      Product Wall

      Thank you for your enquiry. Basically, the epitopes for DE1 and DE2 are complex, conformational epitopes and probably non-linear - and since the immunogen is not a peptide, the amino acid sequence is not known. The information on the recombinan...

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      Unfortunately, we do not know where the epitope for DE1 is on the E glycoprotein. However, we do know that it does not bind recombinant DIII from the pET expression system. This therefore suggests that this antibody will not recognise endogenous domain...

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      Thank you for contacting us.

      We searched on our web page and there are three products that I could recommend to you. They are all three against the IgG2b of mouse and were used for ICC/IF. The species the three products were raised in is diff...

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      Thank you for contacting us.

      ab41349 is a tissue culture supernatant therefore it is normal for it to be reddish as this may be due to the colour of the media. It is likely that there are additional reagents and/or proteins that might oblitera...

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      Thank you for your response.

      Currently, we have antibodies against Dengue virus which detect Type 1 and Type 3 separately.

      If you need any further assistance, please do not hesitate to contact me.

      Thank you for your enquiry and your interest in our products.

      I can confirm that the Western blot results with this antibody (ab41349: Anti-Dengue Virus E glycoprotein antibody [DE1]) have been inconclusive, thus we do not list WB as a tested...

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      Thank you for your enquiry. DE1 (ab41349) and DE2 (ab41696) are monoclonal antibodies from two different fusion experiments but the immunizing protocol is the same for both fusions. As mentioned in the datasheets, although they are both dengue grou...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"