概述

  • 产品名称Anti-delta II tubulin抗体
  • 描述
    兔多克隆抗体to delta II tubulin
  • 经测试应用适用于: WB, ICC/IF, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human delta II tubulin (C terminal). 10 amino acid synthetic peptide.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: Whole serum
  • 纯度Whole antiserum
  • 克隆多克隆
  • 同种型IgG

应用

Our Abpromise guarantee covers the use of ab106658 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000.
ICC/IF Use at an assay dependent concentration. PubMed: 24022482
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Anti-delta II tubulin antibody 图像

  • Figure to show Immunostaining of CCP5 enzymatic activity toward polymerized tubulin. Polymerized microtubules from MDA-MB-231 cells were prepared on plates using microtubule-stabilizing buffer containing 5 μm paclitaxel in the absence of GTP. For immunostaining with tyrosinated tubulin (ab48389) and Δ2-tubulin antibodies (ab106658), microtubules were treated with carboxypeptidase A1 (40 ng/ml) to convert tyrosinated into detyrosinated tubulin. After washes, microtubules were incubated with CCP5 or control eluate for 2 h and then processed for immunocytochemistry.

Anti-delta II tubulin antibody (ab106658)参考文献

This product has been referenced in:
  • Berezniuk I  et al. Cytosolic Carboxypeptidase 5 Removes a- and ?-Linked Glutamates from Tubulin. J Biol Chem 288:30445-53 (2013). ICC/IF ; Human . Read more (PubMed: 24022482) »

See 1 Publication for this product

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