概述

  • 产品名称
    Anti-delta 2 Catenin抗体
    参阅全部 delta 2 Catenin 一抗
  • 描述
    小鼠单克隆抗体to delta 2 Catenin
  • 经测试应用
    适用于: WB, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1081-1191 of Human delta 2 Catenin

  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

性能

应用

Our Abpromise guarantee covers the use of ab54578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 133 kDa.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Functions as a transcriptional activator when bound to ZBTB33 (By similarity). May be involved in neuronal cell adhesion and tissue morphogenesis and integrity by regulating adhesion molecules.
  • 组织特异性
    Predominantly expressed in brain.
  • 序列相似性
    Belongs to the beta-catenin family.
    Contains 9 ARM repeats.
  • 翻译后修饰
    O-glycosylated.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Nucleus. Cell junction > adherens junction.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ARM-repeat protein antibody
    • Catenin (cadherin associated protein) delta 2 antibody
    • catenin (cadherin-associated protein), delta 2 (neural plakophilin-related arm-repeat protein) antibody
    • Catenin delta 2 antibody
    • Catenin delta-2 antibody
    • CTND2_HUMAN antibody
    • CTNND 2 antibody
    • Ctnnd2 antibody
    • Delta catenin antibody
    • Delta-catenin antibody
    • GT 24 antibody
    • GT24 antibody
    • Neural plakophilin related arm repeat protein antibody
    • Neural plakophilin related armadillo repeat protein antibody
    • Neural plakophilin-related ARM-repeat protein antibody
    • Neural plakophilin-related, Neurojungin antibody
    • Neurojungin antibody
    • NPRAP antibody
    • T cell delta catenin antibody
    see all

图片

  • delta 2 Catenin antibody (ab54578) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human stomach.


  • Predicted band size : 133 kDa
  • ICC/IF image of ab54578 stained SKNSH cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54578, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Overlay histogram showing SH-SY5Y cells stained with ab54578 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54578, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

文献

This product has been referenced in:
  • Zhang H  et al. Overexpression of d-catenin is associated with a malignant phenotype and poor prognosis in colorectal cancer. Mol Med Rep 12:4259-65 (2015). WB . Read more (PubMed: 26062780) »
  • Kim H  et al. d-Catenin promotes E-cadherin processing and activates ß-catenin-mediated signaling: implications on human prostate cancer progression. Biochim Biophys Acta 1822:509-21 (2012). IHC-P ; Human . Read more (PubMed: 22261283) »

See all 2 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (lung adenoma)
Specification
lung adenoma
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
Yes - PBST
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Oct 03 2012

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (lung adenoma)
Specification
lung adenoma
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
Yes - PBST
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Oct 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (endometrial adenocarcinoma)
Loading amount
25 µg
Specification
endometrial adenocarcinoma
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Sep 24 2012

Application
Western blot
Sample
Mouse Cell lysate - whole cell (lung cancer primary tumor)
Loading amount
25 µg
Specification
lung cancer primary tumor
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Sep 24 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (prostate)
Specification
prostate
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No
Username

Abcam user community

Verified customer

提交于 Jul 04 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (prostate)
Loading amount
50 µg
Specification
prostate
Gel Running Conditions
Reduced Denaturing (8% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 04 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册