重组Anti-delta 1 Catenin/CAS (phospho Y228)抗体[E205] (ab32403)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E205] to delta 1 Catenin/CAS (phospho Y228)
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-delta 1 Catenin/CAS (phospho Y228)抗体[E205]
参阅全部 delta 1 Catenin/CAS 一抗 -
描述
兔单克隆抗体[E205] to delta 1 Catenin/CAS (phospho Y228) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human delta 1 Catenin/CAS (phospho Y228).
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阳性对照
- WB: HeLa whole cell lysate (ab150035). IHC-P: Breast carcinoma.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
E205 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32403于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/5000 - 1/20000. Detects a band of approximately 115 kDa (predicted molecular weight: 108 kDa).
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IHC-P |
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/5000 - 1/20000. Detects a band of approximately 115 kDa (predicted molecular weight: 108 kDa). |
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage. -
组织特异性
Expressed in vascular endothelium. -
序列相似性
Belongs to the beta-catenin family.
Contains 10 ARM repeats. -
结构域
A possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted. -
翻译后修饰
Phosphorylated by protein-tyrosine kinases. Dephosphorylated by PTPRJ. -
细胞定位
Cytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 1500 Human
- Entrez Gene: 12388 Mouse
- Entrez Gene: 311163 Rat
- Omim: 601045 Human
- SwissProt: O60716 Human
- SwissProt: P30999 Mouse
- Unigene: 166011 Human
- Unigene: 35738 Mouse
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别名
- Cadherin associated Src substrate antibody
- Cadherin-associated Src substrate antibody
- CAS antibody
see all
图片
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All lanes : Anti-delta 1 Catenin/CAS (phospho Y228) antibody [E205] (ab32403)
Lane 1 : HeLa cell lysate, untreated
Lane 2 : HeLa cell lysate, treated with Pervanandate
Lane 3 : HeLa cell lysate, treated with Phosphatase
Predicted band size: 108 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted? -
Ab32403, at a 1/50 dilution, staining delta 1 Catenin/CAS in paraffin embedded breast carcinoma tissue sections by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab32403 被引用在 3 文献中.
- Park SY et al. Glycoprotein 90K Promotes E-Cadherin Degradation in a Cell Density-Dependent Manner via Dissociation of E-Cadherin-p120-Catenin Complex. Int J Mol Sci 18:N/A (2017). PubMed: 29207493
- Surana R et al. IL4 limits the efficacy of tumor-targeted antibody therapy in a murine model. Cancer Immunol Res 2:1103-12 (2014). WB . PubMed: 25204776
- Ma LW et al. Phosphorylated p120-catenin expression has predictive value for oral cancer progression. J Clin Pathol 65:315-9 (2012). PubMed: 22259181