Application
Flow Cytometry
Fixation
Methanol
Permeabilization
Yes - 0.5% v/v tween 20 in PBS
Sample
Mouse Cell (mouse embryonic stem cells)
Specification
mouse embryonic stem cells
Gating Strategy
live cells in fsc vs ssc
Preparation
Cell harvesting/tissue preparation method: cell dissociation buffer
Sample buffer: 5% fish skin gelatin
Sample buffer: 5% fish skin gelatin
Other product details
Incubation time
30 minute(s) · Temperature: 4°C · Diluent: 5% fish skin gelatin
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rabbit PE
Host species: Goat
Clonality: Polyclonal
Conjugation: Phycoerythrin
Host species: Goat
Clonality: Polyclonal
Conjugation: Phycoerythrin
Dilution
1/200
Additional data
Additional Notes
Purple plot is secondary antibody only, green is WT mES cell line shown by WB to express delta-catenin, pink is mutant mES cell line shown by WB to express reduced delta-catenin compared to WT mES cells.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Apr 30 2014