概述

  • 产品名称Anti-DEK抗体
    参阅全部 DEK 一抗
  • 描述
    兔多克隆抗体to DEK
  • 经测试应用适用于: IP, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    9aa synthetic peptide from the region between aa 178-198 of Human DEK

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Stabilization buffer
    Preservative: 0.02% Sodium Azide
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab74975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent dilution.
WB 1/500. Predicted molecular weight: 43 kDa.

靶标

  • 功能Involved in chromatin organization.
  • 组织特异性Ubiquitous. Expressed at relatively high levels.
  • 疾病相关A chromosomal aberration involving DEK is found in a subset of acute myeloid leukemia (AML); also known as acute non-lymphocytic leukemia (PubMed:1549122). Translocation t(6;9)(p23;q34) with NUP214/CAN (PubMed:1549122). It results in the formation of a DEK-NUP214 fusion gene (PubMed:1549122).
  • 序列相似性Contains 1 SAP domain.
  • 翻译后修饰Phosphorylated by CK2. Phosphorylation fluctuates during the cell cycle with a moderate peak during G(1) phase, and weakens the binding of DEK to DNA.
  • 细胞定位Nucleus. Enriched in regions where chromatin is decondensed or sparse in the interphase nuclei.
  • Information by UniProt
  • 数据库链接
  • 别名
    • D6S231E antibody
    • Dek antibody
    • DEK gene antibody
    • DEK oncogene antibody
    • DEK oncogene DNA binding antibody
    • DEK_HUMAN antibody
    • Protein DEK antibody
    see all

Anti-DEK antibody (ab74975)参考文献

This product has been referenced in:
  • Sandén C  et al. The DEK oncoprotein binds to highly and ubiquitously expressed genes with a dual role in their transcriptional regulation. Mol Cancer 13:215 (2014). CHIPseq ; Human . Read more (PubMed: 25216995) »

See 1 Publication for this product

Product Wall

According to our records, ab74975 was proving difficult to use in Western blot and we were in contact in order to help resolve the issue.
Looking at our correspondence, it appears that we are awaiting more details in order to help us better unders...

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Thank you for contacting us.
I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
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Abreviews
Application ChIP
Sample Human Cell lysate - nuclear (Leukemia cell line)
Specification Leukemia cell line
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Detection step Other
Positive control Nuclear lysate of untreated U937 cells.
Username

Abcam user community

Verified customer

提交于 Jan 21 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (testis)
Specification testis
Fixative Formaldehyde
Permeabilization Yes - Triton X-100 0,5%
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Jan 16 2012

Thank you very much for your interest in ab74975. To our knowledge, ab74975 has not been tested in ChIP. I can also offer a discount off a future purchase if you buy ab74975 now, test it in ChIP and submit feedback to us in the form of an Abreview. It ...

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Merci pour ces précisions. En effet, l'anti-DEK ab83099 est également un bon alternatif. Le numéro de commande de remplacement gratuit (une unité de ab83099) est : *******. Vous recevrez prochainement un mail de confirmation comprenant les détails d'ex...

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Je parle effectivement français. Je suis désolé d'apprendre que les 2 anti-DEK ab22826 et ab76975 que vous avez testés ne fonctionnent pas comme indiqué sur les fiches techniques respectives. Votre protocole me semble bon, je crains que vous ayez r...

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Thank you for taking time to complete our questionnaire and for contacting us. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spe...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (knees - sections prepared from the whole knee)
Specification knees - sections prepared from the whole knee
Fixative Paraformaldehyde
Permeabilization Yes - 0.05% Triton X-100
Blocking step Serum as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"