Anti-DEGS1抗体(ab80654)

概述

  • 产品名称Anti-DEGS1抗体
    参阅全部 DEGS1 一抗
  • 描述
    兔多克隆抗体to DEGS1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Rabbit, Goat, Horse, Guinea pig, Cow, Cat, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MGSRVSREDF EWVYTDQPHA DRRREILAKY PEIKSLMKPD PNLIWIIIMM) of human DEGS1 (NP_003667)

  • 阳性对照
    • Fetal muscle lysate

性能

应用

Our Abpromise guarantee covers the use of ab80654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 相关性DEGS1 is a member of the membrane fatty acid desaturase family which is responsible for inserting double bonds into specific positions in fatty acids. It contains three His containing consensus motifs that are characteristic of a group of membrane fatty acid desaturases. It has sphingolipid-delta-4-desaturase activity and converts D-erythro-sphinganine to D-erythro-sphingosine (E-sphing-4-enine).
  • 细胞定位Endoplasmic reticulum membrane; Multi pass membrane protein.
  • 数据库链接
  • 别名
    • Degenerative spermatocyte homolog 1 antibody
    • Degenerative spermatocyte homolog 1 lipid desaturase antibody
    • Degenerative spermatocyte homolog lipid desaturase antibody
    • DEGS 1 antibody
    • DEGS antibody
    • Des 1 antibody
    • DES1 antibody
    • Dihydroceramide desaturase antibody
    • FADS7 antibody
    • Membrane fatty acid (lipid) desaturase antibody
    • Membrane lipid desaturase antibody
    • MGC5079 antibody
    • MIG15 antibody
    • Migration inducing gene 15 protein antibody
    • MLD antibody
    • Sphingolipid delta 4 desaturase antibody
    • Sphingolipid delta(4) desaturase DES1 antibody
    see all

Anti-DEGS1 antibody 图像

  • Anti-DEGS1 antibody (ab80654) at 1 µg/ml + fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 38 kDa
    Observed band size : 38 kDa

Anti-DEGS1 antibody (ab80654)参考文献

ab80654 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (Macrophage cells)
Loading amount 50 µg
Specification Macrophage cells
Gel Running Conditions Non-reduced Denaturing (10% precast polyacrylamide gel)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Jun 12 2012

I'm happy to help! Best of luck in your experiments!



***Regarding your question, I recommend diluting the antibody in TBST + 1% BSA for incubation with the membrane***

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"