Deferoxamine mesylate,Iron chelator (ab120727)
Key features and details
- Iron chelator
- CAS Number: 138-14-7
- Soluble in water to 100 mM
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
Deferoxamine mesylate,Iron chelator -
描述
Iron chelator -
别名
- desferal
- desferrioxamine B
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生物学描述
Iron chelator. Blood-brain barrier permeable. Displays a number of biological actions such as immunomodulation, inhibition of inflammation and atherosclerosis, and reduction of neuronal death.
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CAS编号
138-14-7 -
化学结构
性能
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化学名称
N4-[5-[[4-5-(Acetylhydroxyamino)pentyl]-1,4-dioxobutyl]hydroxyaminopentyl-N1-(5-aminopentyl)-N1-hydroxybutanediamide methanesulfonate -
分子量
656.79 -
分子式
C25H48N6O8.CH3SO3H -
PubChem识别号
62881 -
存放说明
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解度概述
Soluble in water to 100 mM -
处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CC(=O)N(CCCCCNC(=O)CCC(=O)N(CCCCCNC(=O)CCC(=O)N(CCCCCN)O)O)O.CS(=O)(=O)O -
来源
Synthetic
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab120727于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Functional Studies |
Use at an assay dependent concentration.
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说明 |
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Functional Studies
Use at an assay dependent concentration. |
图片
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2D chemical structure image of ab120727, Deferoxamine mesylate, Iron chelator
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Titration of HeLa-DFO extract within the working range of the assay. Background subtracted data from duplicate measurements are plotted. To induce HIF1 alpha protein levels, HeLa cells were treated with 500 µM DFO (ab120727) for 24 hours.
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Dose-dependent induction of HIF1 alpha in HeLa cells by DFO (ab120727). HeLa cells were cultured in 96-well tissue culture plates and were either untreated or exposed to varying dose of DFO for 24 hours. Raw data with standard deviation is plotted from triplicate measurements.
Dose-dependent induction of HIF1 alpha in HeLa cells by DFO (ab120727). HeLa cells were cultured in 96-well tissue culture plates and were either untreated or exposed to varying dose of DFO for 24 hours. Raw data with standard deviation is plotted from triplicate measurements. -
Comparison of HIF1 alpha expression in HeLa cell extracts (with and without DFO treatment) by SimpleStep ELISA (barchart) and western blot (top). Background subtracted OD450 nm data from three loading concentrations are shown. The HIF1 alpha detector antibody was used to blot the same lysates as analyzed by SimpleStep ELISA (40 µg loaded/lane). The GAPDH blot is included to show the relative loads of each lysate. In the HeLa cell line, DFO treatment is required to detect HIF1 alpha protein by both SimpleStep ELISA and western blot.
Comparison of HIF1 alpha expression in HeLa cell extracts (with and without DFO treatment) by SimpleStep ELISA (barchart) and western blot (top). Background subtracted OD450 nm data from three loading concentrations are shown. The HIF1 alpha detector antibody was used to blot the same lysates as analyzed by SimpleStep ELISA (40 µg loaded/lane). The GAPDH blot is included to show the relative loads of each lysate. In the HeLa cell line, DFO treatment is required to detect HIF1 alpha protein by both SimpleStep ELISA and western blot.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (10)
ab120727 被引用在 10 文献中.
- Henning Y et al. Hypoxia aggravates ferroptosis in RPE cells by promoting the Fenton reaction. Cell Death Dis 13:662 (2022). PubMed: 35906211
- You JH et al. PGRMC1-dependent lipophagy promotes ferroptosis in paclitaxel-tolerant persister cancer cells. J Exp Clin Cancer Res 40:350 (2021). PubMed: 34749765
- Tadokoro T et al. Mitochondria-dependent ferroptosis plays a pivotal role in doxorubicin cardiotoxicity. JCI Insight 5:N/A (2020). PubMed: 32376803
- Rakshit J et al. Iron chelator Deferoxamine protects human neuroblastoma cell line SH-SY5Y from 6-Hydroxydopamine-induced apoptosis and autophagy dysfunction. J Trace Elem Med Biol 57:126406 (2020). PubMed: 31570251
- Sugiyama A et al. xCT inhibitor sulfasalazine depletes paclitaxel-resistant tumor cells through ferroptosis in uterine serous carcinoma. Oncol Lett 20:2689-2700 (2020). PubMed: 32782585
- Fu C et al. The transcription factor ZFHX3 is crucial for the angiogenic function of hypoxia-inducible factor 1a in liver cancer cells. J Biol Chem 295:7060-7074 (2020). PubMed: 32277050
- Nishimura K et al. Type 3 iodothyronine deiodinase is expressed in human induced pluripotent stem cell derived cardiomyocytes. Life Sci 203:276-281 (2018). PubMed: 29684447
- Yao Q et al. Mesoporous silicate nanoparticles/3D nanofibrous scaffold-mediated dual-drug delivery for bone tissue engineering. J Control Release 279:69-78 (2018). PubMed: 29649529
- Furukawa T et al. Chronic diazepam administration increases the expression of Lcn2 in the CNS. Pharmacol Res Perspect 5:e00283 (2017). PubMed: 28596835
- Sias SR & Ingraham JL Isolation and analysis of mutants of Pseudomonas aeruginosa unable to assimilate nitrate. Arch Microbiol 122:263-70 (1979). PubMed: 120727