概述

  • 产品名称Anti-DEF6抗体
    参阅全部 DEF6 一抗
  • 描述
    小鼠单克隆抗体to DEF6
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-337 of Human DEF6

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab57228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
WB
  • 应用说明WB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which plays a role in the activation of Rho GTPases RAC1, RhoA and CDC42. Can regulate cell morphology in cooperation with activated RAC1. Plays a role in Th2 (T helper cells) development and/or activation, perhaps by interfering with ZAP-70 signaling.
    • 组织特异性Broadly expressed in the immune system and can be detected in T and B-cells.
    • 序列相似性Contains 1 PH domain.
    • 结构域The PH domain is essential for phosphatidylinositol 3,4,5-trisphosphate binding.
    • 翻译后修饰Tyrosine-phosphorylated by tyrosine-protein kinase LCK in response to T-cell activation.
    • 细胞定位Cytoplasm. Cell membrane. Nucleus. Recruited to the plasma membrane upon binding phosphatidylinositol 3,4,5-trisphosphate.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DEF-6 antibody
      • Def6 antibody
      • DEFI6_HUMAN antibody
      • Differentially expressed in FDCP 6 homolog antibody
      • Differentially expressed in FDCP 6 homologue mouse antibody
      • IBP antibody
      • IRF4-binding protein antibody
      • Swap70 like adapter of T cells antibody
      see all

    Anti-DEF6 antibody 图像

    • Western blot against tagged recombinant protein immunogen using ab57228 DEF6 antibody at 1ug/ml. Predicted band size of immunogen is 63 kDa

    Anti-DEF6 antibody (ab57228)参考文献

    ab57228 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (vero)
    Loading amount 20000 cells
    Specification vero
    Treatment cells expressing recombitant DEF6
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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    提交于 Jun 25 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"