Anti-Deep Orange抗体(ab21872)

概述

  • 产品名称
    Anti-Deep Orange抗体
  • 描述
    小鼠多克隆抗体to Deep Orange
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Drosophila melanogaster
  • 免疫原

    Fusion protein:

    AKETASRPSDSKIRRKLWLRIAYHDIKGTNDVKKALNLLKECDLLRIEDL LPFFADFEKIDNFKEAICDALRDYNQRIQELQREMAETTEQTDRVTAELQ

    , corresponding to amino acids 771/870 of Drosophila melanogaster Deep Orange .

  • 常规说明

    Produced from outbred CD1 mice

     

    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    Constituents: 50% Glycerol
  • 纯度
    Whole antiserum
  • Primary antibody说明
    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab21872 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 115 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

靶标

  • 相关性
    Deep Orange is required for the biogenesis of eye pigment granules. It plays a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. Deep Orange is expressed both maternally and zygotically in all stages of development. Highest expression is in third larval instar. It is a member of the 'granule group' of eye color genes as mutants affect deposition in pigment granules of two types of pigments, the ommochromes and drosopterins.
  • 细胞定位
    Cytoplasmic; peripheral membrane protein associated with late endosomes/lysosomes.
  • 数据库链接
    • 别名
      • Deep orange protein antibody
      • dor antibody
      • dor1 antibody
      • Vacuolar protein sorting 18 antibody

    图片

    • All lanes : Anti-Deep Orange antibody (ab21872) at 1/1000 dilution

      Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
      Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug

      Secondary
      Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

      Predicted band size : 115 kDa

    实验方案

    文献

    ab21872 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Application
    Western blot
    Sample
    Fruit fly (Drosophila melanogaster) Tissue lysate - whole (Full larvae)
    Loading amount
    40 µg
    Specification
    Full larvae
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    提交于 Jan 18 2012

    Thank you for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a f...

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    Thank you for your reply. The antibody vial seems to be damaged somehow, maybe during storage or shipping. I am very sorry for this inconvenience. I do not know what exactly has happened. I suggest therefore that we do replace this vial for you. We hav...

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    Thank you for contacting us. I am sorry to hear you are experiencing difficulties with this antibody. We had so far no complaints about ab21872. We however take everyproduct complaint very seriously, and investigate every product that we feel may not b...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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