Anti-Dectin-1抗体[GE2] - Low endotoxin, Azide free (ab82888)

概述

  • 产品名称
    Anti-Dectin-1抗体[GE2] - Low endotoxin, Azide free
    参阅全部 Dectin-1 一抗
  • 描述
    小鼠单克隆抗体[GE2] to Dectin-1 - Low endotoxin, Azide free
  • 特异性
    Human beta Glucagon Receptor has two major (ßGR-A and ßGR-B) and several minor isoforms. ßGR-A and ßGR-B differ by the presence and absence, respectively, of a stalk region. ab82888 (clone GE2) recognizes both isoform A and B.
  • 经测试应用
    适用于: Inhibition Assay, Flow Cytmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Tissue, cells or virus corresponding to Human Dectin-1.

  • 阳性对照
    • Human peripheral blood monocytes

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 纯化说明
    Endotoxin level: <0.01EU/ug
  • 克隆
    单克隆
  • 克隆编号
    GE2
  • 骨髓瘤
    Sp2
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab82888 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Inhibition Assay Use at an assay dependent concentration.
Flow Cyt 1/25. Use 10µl of the suggested working dilution to label 106 cells in 100µl.



ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation.
  • 组织特异性
    Highly expressed in peripheral blood leukocytes and dendritic cells. Detected in spleen, bone marrow, lung, muscle, stomach and placenta.
  • 疾病相关
    Defects in CLEC7A may be a cause of familial candidiasis type 4 (CANDF4) [MIM:613108]. It is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans.
  • 序列相似性
    Contains 1 C-type lectin domain.
  • 翻译后修饰
    Phosphorylated on tyrosine residues in response to glucan binding.
  • 细胞定位
    Cytoplasm and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Beta GR antibody
    • Beta-glucan receptor antibody
    • BGR antibody
    • C type calcium dependent carbohydrate recognition domain lectin superfamily member 12 antibody
    • C type lectin domain family 7 member a antibody
    • C-type lectin domain family 7 member A antibody
    • C-type lectin superfamily member 12 antibody
    • CANDF4 antibody
    • CLC7A_HUMAN antibody
    • CLEC7A antibody
    • CLEC7A protein antibody
    • Clecsf12 antibody
    • DC-associated C-type lectin 1 antibody
    • Dectin 1 antibody
    • DECTIN 1 receptor antibody
    • Dectin-1 antibody
    • Dectin1 antibody
    • Dendritic cell associated C type lectin 1 antibody
    • Dendritic cell-associated C-type lectin 1 antibody
    • Lectin like receptor 1 antibody
    • Putative transmembrane protein dectin 1 antibody
    see all

图片

  • Flow cytometry analysis of A549 cells, staining beta Glucan Receptor (green) with ab82888.

    Cells were infected with swollen conidia of A. fumigatus 13073 at an MOI of 10 or incubated with PBS (Control) for 30 min. Subsequently, the cells were stained with isotype (IC) antibody or the primary antibody and analyzed by flow cytometry. A PE-conjugated goat anti-mouse IgG was used as the secondary antibody.

文献

This product has been referenced in:
  • Bao Z  et al. Evidence for the involvement of cofilin in Aspergillus fumigatus internalization into type II alveolar epithelial cells. BMC Microbiol 15:161 (2015). Read more (PubMed: 26268695) »
  • Kyrmizi I  et al. Corticosteroids block autophagy protein recruitment in Aspergillus fumigatus phagosomes via targeting dectin-1/Syk kinase signaling. J Immunol 191:1287-99 (2013). Read more (PubMed: 23817424) »

See all 3 Publications for this product

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