Anti-Dectin-1抗体[2A11] (FITC) (ab21646)

概述

  • 产品名称
    Anti-Dectin-1抗体[2A11] (FITC)
    参阅全部 Dectin-1 一抗
  • 描述
    大鼠单克隆抗体[2A11] to Dectin-1 (FITC)
  • 偶联物
    FITC. Ex: 493nm, Em: 528nm
  • 经测试应用
    适用于: Flow Cytmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Recombinant full length protein corresponding to Dectin-1.

  • 常规说明


    This antibody inhibits the binding of zymosan to macrophages via the beta Glucan Receptor.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Protein G purified
  • Primary antibody说明
    This antibody inhibits the binding of zymosan to macrophages via the beta Glucan Receptor.
  • 克隆
    单克隆
  • 克隆编号
    2A11
  • 骨髓瘤
    Y3/Ag1.2.3
  • 同种型
    IgG2b
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab21646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 10µl for 106 cells.

Use 10µl for 106 cells in 100µl.





ab136125-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation.
  • 组织特异性
    Highly expressed in peripheral blood leukocytes and dendritic cells. Detected in spleen, bone marrow, lung, muscle, stomach and placenta.
  • 疾病相关
    Defects in CLEC7A may be a cause of familial candidiasis type 4 (CANDF4) [MIM:613108]. It is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans.
  • 序列相似性
    Contains 1 C-type lectin domain.
  • 翻译后修饰
    Phosphorylated on tyrosine residues in response to glucan binding.
  • 细胞定位
    Cytoplasm and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Beta GR antibody
    • Beta-glucan receptor antibody
    • BGR antibody
    • C type calcium dependent carbohydrate recognition domain lectin superfamily member 12 antibody
    • C type lectin domain family 7 member a antibody
    • C-type lectin domain family 7 member A antibody
    • C-type lectin superfamily member 12 antibody
    • CANDF4 antibody
    • CLC7A_HUMAN antibody
    • CLEC7A antibody
    • CLEC7A protein antibody
    • Clecsf12 antibody
    • DC-associated C-type lectin 1 antibody
    • Dectin 1 antibody
    • DECTIN 1 receptor antibody
    • Dectin-1 antibody
    • Dectin1 antibody
    • Dendritic cell associated C type lectin 1 antibody
    • Dendritic cell-associated C-type lectin 1 antibody
    • Lectin like receptor 1 antibody
    • Putative transmembrane protein dectin 1 antibody
    see all

图片

  • Flow cytometry staining of mouse peripheral blood granulocytes with ab21646.

文献

ab21646 has not yet been referenced specifically in any publications.

客户评价及客户问答


Information we have from the clone originators indicates the antibody recognises both isoforms.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Mouse Cell (RAW and J774 murine macrophages)
Specification
RAW and J774 murine macrophages
Preparation
Cell harvesting/tissue preparation method: routine cell culture --> scraped, washed
Sample buffer: PBS + FBS (3%)
Fixation
None
Permeabilization
No
Gating Strategy
Size gating of murine RAW cells
Username

Abcam user community

Verified customer

提交于 May 23 2008

Thank you for your enquiry. The antibody is specific for Dectin-1. This is the only antibody we carry for this particular target. The references listed on the datasheet are general references pertaining to the target of the antibody. They are n...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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