概述

  • 产品名称Anti-Dectin-1抗体[2A11]
    参阅全部 Dectin-1 一抗
  • 描述
    大鼠单克隆抗体[2A11] to Dectin-1
  • 经测试应用适用于: IP, IHC-Fr, Flow Cytmore details
    不适用于: IHC-P or IHC-R
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Tissue, cells or virus corresponding to Dectin-1.

  • 常规说明


    ab53427 inhibits the binding of zymosan to macrophages via the beta glucan receptor. Fusion Partners: Spleen cells from immunised Fischer rats were fused with cells of the rat Y3 myeloma cell line.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
  • Primary antibody说明ab53427 inhibits the binding of zymosan to macrophages via the beta glucan receptor. Fusion Partners: Spleen cells from immunised Fischer rats were fused with cells of the rat Y3 myeloma cell line.
  • 克隆单克隆
  • 克隆编号2A11
  • 同种型IgG2b
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab53427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

The epitope recognised by 2A11 is sensitive to H2O2 treatment, therefore quenching with 0.3% H2O2 should be performed after incubation with the primary antibody.

Flow Cyt 1/10 - 1/50.

Use 10µl of the suggested working dilution to label 106 cells in 100µl.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

  • 应用说明Is unsuitable for IHC-P or IHC-R.
  • 靶标

    • 功能Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation.
    • 组织特异性Highly expressed in peripheral blood leukocytes and dendritic cells. Detected in spleen, bone marrow, lung, muscle, stomach and placenta.
    • 疾病相关Defects in CLEC7A may be a cause of familial candidiasis type 4 (CANDF4) [MIM:613108]. It is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans.
    • 序列相似性Contains 1 C-type lectin domain.
    • 翻译后修饰Phosphorylated on tyrosine residues in response to glucan binding.
    • 细胞定位Cytoplasm and Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Beta GR antibody
      • Beta-glucan receptor antibody
      • BGR antibody
      • C type calcium dependent carbohydrate recognition domain lectin superfamily member 12 antibody
      • C type lectin domain family 7 member a antibody
      • C-type lectin domain family 7 member A antibody
      • C-type lectin superfamily member 12 antibody
      • CANDF4 antibody
      • CLC7A_HUMAN antibody
      • CLEC7A antibody
      • CLEC7A protein antibody
      • Clecsf12 antibody
      • DC-associated C-type lectin 1 antibody
      • Dectin 1 antibody
      • DECTIN 1 receptor antibody
      • Dectin-1 antibody
      • Dectin1 antibody
      • Dendritic cell associated C type lectin 1 antibody
      • Dendritic cell-associated C-type lectin 1 antibody
      • Lectin like receptor 1 antibody
      • Putative transmembrane protein dectin 1 antibody
      see all

    Anti-Dectin-1 antibody [2A11] 图像

    • Staining with Rat anti mouse beta Glucan Receptor. (ab53427).

    Anti-Dectin-1 antibody [2A11] (ab53427)参考文献

    This product has been referenced in:
    • Seifert L  et al. Dectin-1 Regulates Hepatic Fibrosis and Hepatocarcinogenesis by Suppressing TLR4 Signaling Pathways. Cell Rep 13:1909-21 (2015). Flow Cyt ; Mouse . Read more (PubMed: 26655905) »

    See 1 Publication for this product

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    Thank you for your inquiry.

    I am sorry the epitope of this antibody hasn't been determined yet. The immunogen of this antibody corresponds to recombinant beta Glucan Receptor protein.

    I hope this information is helpful to you. Ple...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"