Anti-DECR1抗体(ab76630)

概述

  • 产品名称Anti-DECR1抗体
    参阅全部 DECR1 一抗
  • 描述
    小鼠单克隆抗体to DECR1
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey
  • 免疫原

    Recombinant fragment: NVIQPGPIKT KGAFSRLDPT GTFEKEMIGR IPCGRLGTVE ELANLAAFLC SDYASWINGA VIKFDGGEEV LISGEFNDLR KVTKEQWDTI EELIRKTKGS, corresponding to amino acids 236-336 of Human DECR1 (NP_001350) with tag.

  • 阳性对照
    • HepG2 cell lysate; Recombinant tagged human DECR1 fragment.

性能

应用

Our Abpromise guarantee covers the use of ab76630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 36 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 功能Auxiliary enzyme of beta-oxidation. It participates in the metabolism of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions. Catalyzes the NADP-dependent reduction of 2,4-dienoyl-CoA to yield trans-3-enoyl-CoA.
  • 组织特异性Heart = liver = pancreas > kidney >> skeletal muscle = lung.
  • 序列相似性Belongs to the short-chain dehydrogenases/reductases (SDR) family. 2,4-dienoyl-CoA reductase subfamily.
  • 细胞定位Mitochondrion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1200012F07Rik antibody
    • 2 antibody
    • 2,4 dienoyl CoA reductase [NADPH] antibody
    • 2,4 dienoyl CoA reductase 1 antibody
    • 2,4 dienoyl CoA reductase mitochondrial antibody
    • 2,4-dienoyl-CoA reductase, mitochondrial antibody
    • 4 enoyl CoA reductase antibody
    • 4-dienoyl-CoA reductase [NADPH] antibody
    • 4-enoyl-CoA reductase [NADPH] antibody
    • DECR 1 antibody
    • DECR antibody
    • DECR_HUMAN antibody
    • DECR1 antibody
    • Nadph antibody
    • OTTMUSP00000004962 antibody
    • RP23-338J24.3 antibody
    • SDR18C1 antibody
    • Short chain dehydrogenase/reductase family 18C member 1 antibody
    see all

Anti-DECR1 antibody 图像

  • Anti-DECR1 antibody (ab76630) at 1 µg/ml + Recombinant tagged human DECR1 fragment at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 36 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-DECR1 antibody (ab76630) at 1 µg/ml + HepG2 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 36 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)

Anti-DECR1 antibody (ab76630)参考文献

ab76630 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Heart)
Permeabilization Yes - Tween-20
Specification Heart
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 24 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Heart)
Gel Running Conditions Reduced Denaturing (4-12%)
Loading amount 20 µg
Specification Heart
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 19 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"