概述

  • 产品名称Anti-Decorin抗体
    参阅全部 Decorin 一抗
  • 描述
    小鼠单克隆抗体to Decorin
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human, Recombinant Fragment
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-360 of Human Decorin

性能

应用

Our Abpromise guarantee covers the use of ab54728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 20801503

靶标

  • 功能May affect the rate of fibrils formation.
  • 疾病相关Defects in DCN are the cause of congenital stromal corneal dystrophy (CSCD) [MIM:610048]. Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance.
  • 序列相似性Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
    Contains 12 LRR (leucine-rich) repeats.
  • 翻译后修饰The attached glycosaminoglycan chain can be either chondroitin sulfate or dermatan sulfate depending upon the tissue of origin.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Bone proteoglycan II antibody
    • CSCD antibody
    • DCN antibody
    • DCN protein antibody
    • Decorin antibody
    • Decorin proteoglycan antibody
    • Dermatan sulphate proteoglycans II antibody
    • DKFZp686J19238 antibody
    • DSPG 2 antibody
    • DSPG2 antibody
    • PG 40 antibody
    • PG II antibody
    • PG S2 antibody
    • PG-S2 antibody
    • PG40 antibody
    • PGII antibody
    • PGS 2 antibody
    • PGS2 antibody
    • PGS2_HUMAN antibody
    • Proteoglycan core protein antibody
    • SLRR1B antibody
    • Small leucine rich protein 1B antibody
    see all

Anti-Decorin antibody 图像

  • Decorin antibody (ab54728) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human adrenal gland.
  • Western blot against tagged recombinant protein immunogen using ab54728 Decorin antibody at 1ug/ml. Predicted band size of immunogen is 65 kDa

Anti-Decorin antibody (ab54728)参考文献

This product has been referenced in:
  • Soria-Valles C  et al. The anti-metastatic activity of collagenase-2 in breast cancer cells is mediated by a signaling pathway involving decorin and miR-21. Oncogene N/A:N/A (2013). Read more (PubMed: 23851508) »
  • Botfield H  et al. Decorin prevents the development of juvenile communicating hydrocephalus. Brain 136:2842-58 (2013). Read more (PubMed: 23983032) »

See all 5 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Sample Human Cell (fibroblast)
Specification fibroblast
Permeabilization No
Fixative Paraformaldehyde
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提交于 Jun 11 2014

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Dermis)
Specification Dermis
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 19°C
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提交于 Jan 27 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"