The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明ELISA: 1/150 - 1/500.
Coat with 1ug antigen per well. Use AP conjugated anti-sheep for detection.
Is unsuitable for IHC and WB.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能May affect the rate of fibrils formation.
疾病相关Defects in DCN are the cause of congenital stromal corneal dystrophy (CSCD) [MIM:610048]. Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance.
序列相似性Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily. Contains 12 LRR (leucine-rich) repeats.
翻译后修饰The attached glycosaminoglycan chain can be either chondroitin sulfate or dermatan sulfate depending upon the tissue of origin.
细胞定位Secreted > extracellular space > extracellular matrix.