概述

  • 产品名称Anti-DDX6抗体
    参阅全部 DDX6 一抗
  • 描述
    兔多克隆抗体to DDX6
  • 经测试应用适用于: IHC-P, WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a sequence between N terminal residues 1 to 50 of human DDX6 (Swiss-Prot P26196).

  • 阳性对照
    • Whole cell lysate from HeLa cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline, pH 7-8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab70455 was affinity purified using an epitope specific to DDX6 immobilized on solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab70455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/5000 - 1/15000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

  • 相关性DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis and cellular growth and division. In the process of mRNA degradation, DDX6 may play a role in mRNA decapping. It forms a complex with DCP1A, DCP2, EDC3 and EDC4/HEDLS.
  • 细胞定位Cytoplasm; P-body. Note: Processing bodies (PB).
  • 数据库链接
  • 别名
    • ATP dependent RNA helicase DDX6 antibody
    • ATP dependent RNA helicase p54 antibody
    • DDX6 antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 6 antibody
    • DEAD box 6 antibody
    • DEAD box protein 6 antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 6 (RNA helicase 54kD) antibody
    • FLJ36338 antibody
    • HLR2 antibody
    • Oncogene RCK antibody
    • P54 antibody
    • Probable ATP-dependent RNA helicase DDX6 antibody
    • RCK antibody
    see all

Anti-DDX6 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling DDX6 with ab70455 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-DDX6 antibody (ab70455) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg


    Predicted band size : 54 kDa
    Observed band size : 54 kDa
  • 250µg HeLa whole cell lysate treated with ab70455 at 0.04 µg/ml for the Western blot, and at 3 µg/mg lysate for Immunoprecipitation.

Anti-DDX6 antibody (ab70455)参考文献

ab70455 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application CLIP
Sample Human Cell lysate - whole cell (HEK 293T)
Specification HEK 293T
Type iCLIP
Irradiation Energy Level (mJ/cm2): 150
Wavelength (nm): 254
Positive control hnRNP C (sc-15386) bound protein A dynabeads, 0.1 mg/ml (antibody/beads)
Negative control Protein A dynabeads without antibody bound
Immuno-precipitation step Other - Protein A dynabeads
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提交于 Jun 02 2011

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