概述

  • 产品名称Anti-DDX6抗体
    参阅全部 DDX6 一抗
  • 描述
    山羊多克隆抗体to DDX6
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human, Pig
    预测可用于: Mouse, Guinea pig, Dog
  • 免疫原

    Synthetic peptide corresponding to Human DDX6 aa 471-483 (C terminal).
    Sequence:

    C-AEYHSEPVEDEKP


    Database link: P26196

  • 阳性对照
    • Jurkat cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab45869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明Peptide ELISA: Antibody detection limit dilution 1:16,000.
    WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis and cellular growth and division. In the process of mRNA degradation, DDX6 may play a role in mRNA decapping. It forms a complex with DCP1A, DCP2, EDC3 and EDC4/HEDLS.
    • 细胞定位Cytoplasm; P-body. Note: Processing bodies (PB).
    • 数据库链接
    • 别名
      • ATP dependent RNA helicase DDX6 antibody
      • ATP dependent RNA helicase p54 antibody
      • DDX6 antibody
      • DEAD (Asp Glu Ala Asp) box polypeptide 6 antibody
      • DEAD box 6 antibody
      • DEAD box protein 6 antibody
      • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 6 (RNA helicase 54kD) antibody
      • FLJ36338 antibody
      • HLR2 antibody
      • Oncogene RCK antibody
      • P54 antibody
      • Probable ATP-dependent RNA helicase DDX6 antibody
      • RCK antibody
      see all

    Anti-DDX6 antibody 图像

    • All lanes : Anti-DDX6 antibody (ab45869) at 0.3 µg/ml

      Lane 1 : Human Burkitt's lymphoma cell line
      Lane 2 : Human T cell leukemia cells from peripheral blood
      Lane 3 : Human liver hepatocellular carcinoma

      Lysates/proteins at 35 µg per lane.


      Predicted band size : 54 kDa
    • Anti-DDX6 antibody (ab45869) at 0.3 µg/ml + Jurkat cell lysate (35µg protein in RIPA buffer).

      Predicted band size : 54 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)
      Primary incubation was 1 hour. Detected by chemiluminescence.

    Anti-DDX6 antibody (ab45869)参考文献

    This product has been referenced in:
    • Shen X  et al. Identification of novel SHPS-1-associated proteins and their roles in regulation of insulin-like growth factor-dependent responses in vascular smooth muscle cells. Mol Cell Proteomics : (2009). WB ; Pig . Read more (PubMed: 19299420) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"