Anti-DDX58抗体(ab111037)

概述

• 产品名称
  Anti-DDX58抗体
  参阅全部 DDX58 一抗
• 描述
  山羊多克隆抗体to DDX58
• 经测试应用
  适用于: IHC-Pmore details
• 种属反应性
  与反应: Human
  
• 免疫原

  Human DDX58.

• 阳性对照
  • Human brain cortex, placenta and spleen tissues.

性能

• 形式
  Liquid
• 存放说明
  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
• 存储溶液
  pH: 7.30
  Preservative: 0.02% Sodium azide
  Constituents: 0.5% BSA, Tris buffered saline
• Concentration information loading...
• 克隆
  多克隆
• 同种型
  IgG
• 研究领域
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • RNA Processing
  • Other

  • Epigenetics and Nuclear Signaling
  • Chromatin Binding Proteins
  • DNA / RNA binding

  • Immunology
  • Immune System Diseases
  • Antiviral Signaling

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  • Donkey Anti-Goat IgG H&L (HRP) (ab205723)

应用

靶标

• 功能
  Involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). Essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV.
• 组织特异性
  Present in vascular smooth cells (at protein level).
• 序列相似性
  Belongs to the helicase family.
  Contains 2 CARD domains.
  Contains 1 helicase ATP-binding domain.
  Contains 1 helicase C-terminal domain.
• 结构域
  The repressor domain controls homomultimerization and interaction with MAVS.
  The helicase domain is responsible for dsRNA recognition.
  The 2 CARD domains are responsible for interaction with and signaling through MAVS.
  The second CARD domain is the primary site for 'Lys-63'-linked ubiquitination.
• 翻译后修饰
  Isgylated. Conjugated to ubiquitin-like protein ISG15 upon IFN-beta stimulation.
  Ubiquitinated. Undergoes 'Lys-63'-linked ubiquitination. Lys-172 is the critical site for TRIM25-mediated ubiquitination, for MAVS binding and to induce anti-viral signal transduction. Lys-154, Lys-164 and Lys-172 are critical sites for RNF135-mediated ubiquitination. Deubiquitinated by CYLD, a protease that selectively cleaves 'Lys-63'-linked ubiquitin chains.
• 细胞定位
  Cytoplasm. Colocalized with TRIM25 at cytoplasmic perinuclear bodies.

• Target information above from: UniProt accession O95786 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• 数据库链接
  • Entrez Gene: 23586 Human
  • Omim: 609631 Human
  • SwissProt: O95786 Human
  • Unigene: 190622 Human
• 别名
  • Ddx58 antibody
  • DDX58_HUMAN antibody
  • DEAD (Asp Glu Ala Asp) box polypeptide 58 antibody

  • DEAD (Asp Glu Ala Asp/His) box polypeptide antibody
  • DEAD box protein 58 antibody
  • DEAD/H (Asp Glu Ala Asp/His) box polypeptide RIG1 antibody
  • DKFZp434J1111 antibody
  • DKFZp686N19181 antibody
  • FLJ13599 antibody
  • Probable ATP dependent RNA helicase DDX58 antibody
  • Probable ATP-dependent RNA helicase DDX58 antibody
  • Retinoic acid inducible gene 1 protein antibody
  • Retinoic acid-inducible gene 1 protein antibody
  • Retinoic acid-inducible gene I protein antibody
  • RIG I antibody
  • Rig-1 antibody
  • RIG-I antibody
  • RIG1 antibody
  • rigi antibody
  • RLR 1 antibody
  • RNA helicase antibody
  • RNA helicase RIG I antibody
  • SGMRT2 antibody

  see all

实验方案

• Immunohistochemistry protocols