Anti-RIG-I/DDX58抗体(ab111037)
Key features and details
- Goat polyclonal to RIG-I/DDX58
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RIG-I/DDX58抗体
参阅全部 RIG-I/DDX58 一抗 -
描述
山羊多克隆抗体to RIG-I/DDX58 -
宿主
Goat -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human RIG-I/DDX58. NP_055129.2
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阳性对照
- Human brain cortex, placenta and spleen tissues.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab111037于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 3.75 µg/ml.
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说明 |
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IHC-P
Use a concentration of 3.75 µg/ml. |
靶标
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功能
Involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). Essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV. -
组织特异性
Present in vascular smooth cells (at protein level). -
序列相似性
Belongs to the helicase family.
Contains 2 CARD domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
结构域
The repressor domain controls homomultimerization and interaction with MAVS.
The helicase domain is responsible for dsRNA recognition.
The 2 CARD domains are responsible for interaction with and signaling through MAVS.
The second CARD domain is the primary site for 'Lys-63'-linked ubiquitination. -
翻译后修饰
Isgylated. Conjugated to ubiquitin-like protein ISG15 upon IFN-beta stimulation.
Ubiquitinated. Undergoes 'Lys-63'-linked ubiquitination. Lys-172 is the critical site for TRIM25-mediated ubiquitination, for MAVS binding and to induce anti-viral signal transduction. Lys-154, Lys-164 and Lys-172 are critical sites for RNF135-mediated ubiquitination. Deubiquitinated by CYLD, a protease that selectively cleaves 'Lys-63'-linked ubiquitin chains. -
细胞定位
Cytoplasm. Colocalized with TRIM25 at cytoplasmic perinuclear bodies. - Information by UniProt
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数据库链接
- Entrez Gene: 23586 Human
- Omim: 609631 Human
- SwissProt: O95786 Human
- Unigene: 190622 Human
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别名
- Ddx58 antibody
- DDX58_HUMAN antibody
- DEAD (Asp Glu Ala Asp) box polypeptide 58 antibody
see all
图片
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ab111037, at 3.75µg/ml, staining RIG-I/DDX58 in formalin-fixed, paraffin-embedded Human brain cortex tissue by Immunohistochemistry.
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ab111037, at 3.75µg/ml, staining RIG-I/DDX58 in Formalin-fixed, Paraffin-embedded Human Placenta tissue by Immunohistochemistry.
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ab111037, at 3.75µg/ml, staining RIG-I/DDX58 in formalin-fixed, paraffin-embedded Human Spleen tissue by Immunohistochemistry.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab111037 尚未被引用在任何文献中。