产品名称 Anti-DDX56抗体 参阅全部 DDX56 一抗
WB, IP, IHC-P more details
Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide (Human) corresponding to a region between residue 300 and 350 of DDX56 according to NP_061955.1 (GeneID 54606).
HeLa whole cell lysate, 293T whole cell lysate
存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液 Preservative: 0.09% Sodium azide Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8
Concentration information loading...
纯度 Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 62 kDa.
Use at 2-5 µg/mg of lysate.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
功能 May play a role in later stages of the processing of the pre-ribosomal particles leading to mature 60S ribosomal subunits. Has intrinsic ATPase activity.
组织特异性 Detected in heart, brain, liver, pancreas, placenta and lung.
序列相似性 Belongs to the DEAD box helicase family. DDX56/DBP9 subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
翻译后修饰 Phosphorylated upon DNA damage, probably by ATM or ATR.
细胞定位 Nucleus > nucleolus.
Information by UniProt
61 kd nucleolar helicase antibody
ATP-dependent 61 kDa nucleolar RNA helicase antibody
Anti-DDX56 antibody 图像
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX56 antibody (ab115178)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling DDX56 with ab115178 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - Anti-DDX56 antibody (ab115178)
All lanes : Anti-DDX56 antibody (ab115178) at 0.1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg developed using the ECL technique Predicted band size : 62 kDa Exposure time : 30 seconds
Immunoprecipitation - Anti-DDX56 antibody (ab115178)
Detection of DDX56 by Immunoprecipitation analysis in Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded) using ab115178 for IP at 3 ug/mg lysate. Subsequence WB detection was performed using 1 ug/ml ab115178.
Anti-DDX56 antibody (ab115178)参考文献
has not yet been referenced specifically in any publications.
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