Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide (Human) corresponding to a region between residue 300 and 350 of DDX56 according to NP_061955.1 (GeneID 54606).
HeLa whole cell lysate, 293T whole cell lysate
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 62 kDa.
Use at 2-5 µg/mg of lysate.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
May play a role in later stages of the processing of the pre-ribosomal particles leading to mature 60S ribosomal subunits. Has intrinsic ATPase activity.
Detected in heart, brain, liver, pancreas, placenta and lung.
Belongs to the DEAD box helicase family. DDX56/DBP9 subfamily.
Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Nucleus > nucleolus.
Information by UniProt
61 kd nucleolar helicase antibody
ATP-dependent 61 kDa nucleolar RNA helicase antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX56 antibody (ab115178)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling DDX56 with ab115178 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - Anti-DDX56 antibody (ab115178)
All lanes : Anti-DDX56 antibody (ab115178) at 0.1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Developed using the ECL technique Predicted band size : 62 kDa Exposure time : 30 seconds
Immunoprecipitation - Anti-DDX56 antibody (ab115178)
Detection of DDX56 by Immunoprecipitation analysis in Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded) using ab115178 for IP at 3 ug/mg lysate. Subsequence WB detection was performed using 1 ug/ml ab115178.
has not yet been referenced specifically in any publications.
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