Immunoprecipitation of DDX56 (3 µg of antibody per mg of HeLa cell lysate; 20% of the IP loaded). Lane 1-Immunoprecipitation with an anti-DDX56 antibody that recognizes an upstream epitope; Lane 2- Immunoprecipitation with anti-DDX56 antibody (ab115158); Lane 3- Immunoprecipitation with IgG control. Anti-DDX56 antibody (ab115158) used at 1 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 30 sec.
Western blot - DDX56 antibody (ab115158)
All lanes : Anti-DDX56 antibody (ab115158) at 0.1 µg/ml
Lane 1 : HeLa cell lysate at 50 µg Lane 2 : HeLa cell lysate at 15 µg Lane 3 : HeLa cell lysate at 5 µg Lane 4 : 293T cell lysate at 50 µg
Predicted band size : 62 kDa
Exposure time : 3 minutes
Anti-DDX56 antibody (ab115158)参考文献
has not yet been referenced specifically in any publications.