Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within amino acids 497-547 of Human DDX56 (Q9NY93).
HeLa and 293T cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8.
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 62 kDa.
Use at 2-5 µg/mg of lysate.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
May play a role in later stages of the processing of the pre-ribosomal particles leading to mature 60S ribosomal subunits. Has intrinsic ATPase activity.
Detected in heart, brain, liver, pancreas, placenta and lung.
Belongs to the DEAD box helicase family. DDX56/DBP9 subfamily.
Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Nucleus > nucleolus.
Information by UniProt
61 kd nucleolar helicase antibody
ATP-dependent 61 kDa nucleolar RNA helicase antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX56 antibody (ab115158)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling DDX56 with ab115158 at 1/1000 (1µg/ml). Detection: DAB.
Immunoprecipitation - Anti-DDX56 antibody (ab115158)
Immunoprecipitation of DDX56 (3 µg of antibody per mg of HeLa cell lysate; 20% of the IP loaded). Lane 1-Immunoprecipitation with an anti-DDX56 antibody that recognizes an upstream epitope; Lane 2- Immunoprecipitation with anti-DDX56 antibody (ab115158); Lane 3- Immunoprecipitation with IgG control. Anti-DDX56 antibody (ab115158) used at 1 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 30 sec.
Western blot - DDX56 antibody (ab115158)
All lanes : Anti-DDX56 antibody (ab115158) at 0.1 µg/ml Lane 1 : HeLa cell lysate at 50 µg Lane 2 : HeLa cell lysate at 15 µg Lane 3 : HeLa cell lysate at 5 µg Lane 4 : 293T cell lysate at 50 µg Predicted band size: 62 kDa Exposure time: 3 minutes
has not yet been referenced specifically in any publications.
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