Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within amino acids 49-549 of Human DDX52 (NP_008941.2)
Whole cell lysate from HeLa cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8.
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
Is unsuitable for WB.
Belongs to the DEAD box helicase family. DDX52/ROK1 subfamily.
Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Nucleus > nucleolus.
Information by UniProt
ATP dependent RNA helicase ROK1 like antibody
ATP-dependent RNA helicase ROK1-like antibody
Immunoprecipitation - Anti-DDX52 antibody (ab112027)
Immunoprecipitation of DDX52. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Lane 1- DDXR52 was Immunoprecipitated by rabbit anti-DDX52 antibody (ab112027) at 6 µg/mg lysate. Lane 2- DDX52 was also immunoprecipitated by an rabbit anti-DDX52 antibody that recognizes a downstream epitope. Lane 3- Rabbit IgG control Western blot was performed with the same antibody as used in lane 2 at 0.4 mcg/ml. Detection:
Chemiluminescence with an exposure time of 3 seconds.
has not yet been referenced specifically in any publications.
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