概述

  • 产品名称Anti-DDX51抗体
    参阅全部 DDX51 一抗
  • 描述
    兔多克隆抗体to DDX51
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide cprresponding to a region between residues 616 and 666 of human DDX51 (NP_778236.1)

  • 阳性对照
    • HeLa whole cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab72292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

  • 相关性DDX51 belongs to the DEAD box helicase family. It is thought to be an ATP-binding RNA helicase involved in the biogenesis of 60S ribosomal subunits. Helicases are involved in unwinding nucleic acids. The DEAD box helicases are involved in various aspects of RNA metabolism, including nuclear transcription, pre mRNA splicing, ribosome biogenesis, nucleocytoplasmic transport, translation, RNA decay and organellar gene expression.
  • 细胞定位Nuclear, nucleolus.
  • 数据库链接
  • 别名
    • DDX 51 antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 51 antibody
    • DEAD box protein 51 antibody
    • Dead box protein 73D like antibody
    • MGC42193 antibody
    see all

Anti-DDX51 antibody 图像

  • All lanes : Anti-DDX51 antibody (ab72292) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 72 kDa
    Observed band size : 72 kDa
    Additional bands at : 172 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • Detection of DDX51 by Western Blot of Immunprecipitate.
    Lane 1: ab72292, at 1µg/ml, staining DDX51 in HeLa whole cell lysate immunoprecipitated using ab72292 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded).
    Lane 2: Control IgG.

    Detection: Chemiluminescence with an exposure time of 3 minutes.

Anti-DDX51 antibody (ab72292)参考文献

ab72292 has not yet been referenced specifically in any publications.

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