HT1080 cells were fixed with paraformaldehyde permeabilized with Triton (1%) and stained for 1 hr in a 1/100 dilution of the DDX5 antibody and thereafter for 1 hr with a rhodamine conjugated anti-goat secondary. This image was kindly supplied as part of the review submitted by Reinhard Kodym.
Western blot - Anti-DDX5 antibody (ab10261)
ab10261 staining (0.5µg/ml) of A431 cell lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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