概述

  • 产品名称Anti-DDX5抗体
    参阅全部 DDX5 一抗
  • 描述
    山羊多克隆抗体to DDX5
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Dog, Non Human Primates
  • 免疫原

    Synthetic peptide: PMIGYPMPTGYSQ, corresponding to C terminal amino acids 602-614 of Human DDX5.

  • 阳性对照
    • Hela and A431 lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab10261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 71 kDa).Can be blocked with Human DDX5 peptide (ab23200).
IHC-P Use a concentration of 2 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent dilution. See Abreview submitted by Reinhard Kodym.

靶标

  • 功能RNA-dependent ATPase activity. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. May be involved in pre-mRNA splicing.
  • 序列相似性Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • 翻译后修饰Arg-502 is dimethylated, probably to asymmetric dimethylarginine.
  • 细胞定位Nucleus > nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATP dependent RNA helicase DDX5 antibody
    • DDX 5 antibody
    • Ddx5 antibody
    • DDX5_HUMAN antibody
    • DEAD (Asp Glu Ala Asp) box helicase 5 antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 5 antibody
    • DEAD box 5 antibody
    • DEAD box protein 5 antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 5 (RNA helicase, 68kD) antibody
    • G17P1 antibody
    • HELR antibody
    • HLR1 antibody
    • HUMP68 antibody
    • P68 antibody
    • p68 RNA helicase antibody
    • Probable ATP dependent RNA helicase DDX5 antibody
    • Probable ATP-dependent RNA helicase DDX5 antibody
    • RNA helicase p68 antibody
    see all

Anti-DDX5 antibody 图像

  • HT1080 cells were fixed with paraformaldehyde permeabilized with Triton (1%) and stained for 1 hr in a 1/100 dilution of the DDX5 antibody and thereafter for 1 hr with a rhodamine conjugated anti-goat secondary. This image was kindly supplied as part of the review submitted by Reinhard Kodym.


  • Predicted band size : 71 kDa
  • Immunohistochemical analysis of paraffin-embedded Human prostate tissue, staining DDX5 with ab10261 at 2.5 µg/ml. Staining was detected using Alkaline-Phosphatase .

Anti-DDX5 antibody (ab10261)参考文献

This product has been referenced in:
  • Llompart CM  et al. Specific residues of PB2 and PA influenza virus polymerase subunits confer the ability for RNA polymerase II degradation and virus pathogenicity in mice. J Virol 88:3455-63 (2014). Read more (PubMed: 24403580) »
  • Samaan S  et al. The Ddx5 and Ddx17 RNA helicases are cornerstones in the complex regulatory array of steroid hormone-signaling pathways. Nucleic Acids Res N/A:N/A (2013). WB ; Human . Read more (PubMed: 24275493) »

See all 10 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"