概述

  • 产品名称
    Anti-DDX5抗体[2257C3a]
    参阅全部 DDX5 一抗
  • 描述
    小鼠单克隆抗体[2257C3a] to DDX5
  • 经测试应用
    适用于: WB, Dot blotmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant fragment of human DDX5.

  • 阳性对照
    • HeLa whole cell lysate, NIH3T3 whole cell lysate and F2408 whole cell lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 纯化说明
    ab53216 was purified from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 µm membrane.
  • 克隆
    单克隆
  • 克隆编号
    2257C3a
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab53216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
Dot blot Use at an assay dependent dilution.

靶标

  • 功能
    RNA-dependent ATPase activity. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. May be involved in pre-mRNA splicing.
  • 序列相似性
    Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • 翻译后修饰
    Arg-502 is dimethylated, probably to asymmetric dimethylarginine.
  • 细胞定位
    Nucleus > nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATP dependent RNA helicase DDX5 antibody
    • DDX 5 antibody
    • Ddx5 antibody
    • DDX5_HUMAN antibody
    • DEAD (Asp Glu Ala Asp) box helicase 5 antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 5 antibody
    • DEAD box 5 antibody
    • DEAD box protein 5 antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 5 (RNA helicase, 68kD) antibody
    • G17P1 antibody
    • HELR antibody
    • HLR1 antibody
    • HUMP68 antibody
    • P68 antibody
    • p68 RNA helicase antibody
    • Probable ATP dependent RNA helicase DDX5 antibody
    • Probable ATP-dependent RNA helicase DDX5 antibody
    • RNA helicase p68 antibody
    see all

图片



  • Predicted band size : 69 kDa

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: DDX5 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: MCF7 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab53216 observed at 69 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab53216 was shown to specifically react with DDX5 in wild-type cells as signal was lost in DDX5 knockout cells. Wild-type and DDX5 knockout samples were subjected to SDS-PAGE. Ab53216 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/100 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Anti-DDX5 antibody [2257C3a] (ab53216) at 1/100 dilution + F2408 whole cell lysate at 25 µg

    Secondary
    anti-Mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 69 kDa
    Observed band size : 69 kDa
  • Anti-DDX5 antibody [2257C3a] (ab53216) at 1/100 dilution + HeLa whole cell lysate at 25 µg

    Secondary
    anti-Mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 69 kDa
    Observed band size : 69 kDa
  • Anti-DDX5 antibody [2257C3a] (ab53216) at 1/100 dilution + NIH3T3 whole cell lysate at 25 µg

    Secondary
    anti-mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 69 kDa
    Observed band size : 69 kDa

实验方案

文献

This product has been referenced in:
  • Pettersson OJ  et al. DDX6 regulates sequestered nuclear CUG-expanded DMPK-mRNA in dystrophia myotonica type 1. Nucleic Acids Res 42:7186-200 (2014). Read more (PubMed: 24792155) »
  • Yao H  et al. Mediation of CTCF transcriptional insulation by DEAD-box RNA-binding protein p68 and steroid receptor RNA activator SRA. Genes Dev 24:2543-55 (2010). Read more (PubMed: 20966046) »

See all 2 Publications for this product

客户评价及客户问答

There are currently no Customer reviews or Questions for ab53216.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册