概述

  • 产品名称Anti-DDX5抗体[2257C3a]
    参阅全部 DDX5 一抗
  • 描述
    小鼠单克隆抗体[2257C3a] to DDX5
  • 经测试应用适用于: WB, Dot blotmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant fragment of human DDX5.

  • 阳性对照
    • HeLa whole cell lysate, NIH3T3 whole cell lysate and F2408 whole cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明ab53216 was purified from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 µm membrane.
  • 克隆单克隆
  • 克隆编号2257C3a
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab53216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
Dot blot Use at an assay dependent dilution.

靶标

  • 功能RNA-dependent ATPase activity. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. May be involved in pre-mRNA splicing.
  • 序列相似性Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • 翻译后修饰Arg-502 is dimethylated, probably to asymmetric dimethylarginine.
  • 细胞定位Nucleus > nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATP dependent RNA helicase DDX5 antibody
    • DDX 5 antibody
    • Ddx5 antibody
    • DDX5_HUMAN antibody
    • DEAD (Asp Glu Ala Asp) box helicase 5 antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 5 antibody
    • DEAD box 5 antibody
    • DEAD box protein 5 antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 5 (RNA helicase, 68kD) antibody
    • G17P1 antibody
    • HELR antibody
    • HLR1 antibody
    • HUMP68 antibody
    • P68 antibody
    • p68 RNA helicase antibody
    • Probable ATP dependent RNA helicase DDX5 antibody
    • Probable ATP-dependent RNA helicase DDX5 antibody
    • RNA helicase p68 antibody
    see all

Anti-DDX5 antibody [2257C3a] 图像

  • Anti-DDX5 antibody [2257C3a] (ab53216) at 1/100 dilution + F2408 whole cell lysate at 25 µg

    Secondary
    anti-Mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 69 kDa
    Observed band size : 69 kDa
  • Anti-DDX5 antibody [2257C3a] (ab53216) at 1/100 dilution + HeLa whole cell lysate at 25 µg

    Secondary
    anti-Mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 69 kDa
    Observed band size : 69 kDa
  • Anti-DDX5 antibody [2257C3a] (ab53216) at 1/100 dilution + NIH3T3 whole cell lysate at 25 µg

    Secondary
    anti-mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 69 kDa
    Observed band size : 69 kDa

Anti-DDX5 antibody [2257C3a] (ab53216)参考文献

This product has been referenced in:
  • Pettersson OJ  et al. DDX6 regulates sequestered nuclear CUG-expanded DMPK-mRNA in dystrophia myotonica type 1. Nucleic Acids Res 42:7186-200 (2014). Read more (PubMed: 24792155) »
  • Yao H  et al. Mediation of CTCF transcriptional insulation by DEAD-box RNA-binding protein p68 and steroid receptor RNA activator SRA. Genes Dev 24:2543-55 (2010). Read more (PubMed: 20966046) »

See all 2 Publications for this product

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