概述

  • 产品名称Anti-DDX46抗体
    参阅全部 DDX46 一抗
  • 描述
    兔多克隆抗体to DDX46
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis , Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Synthetic peptide corresponding to a region between residue 981 and the C-terminus (residue 1031) of human DDX46 (NP_055644.2)

  • 阳性对照
    • Whole cell lysate from HeLa, 293T or NIH3T3 cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab72083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 117 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

Anti-DDX46 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling DDX46 with ab72083 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-DDX46 antibody (ab72083) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg
    Lane 5 : Whole cell lysate from NIH 3T3 cells at 50 µg


    Predicted band size : 117 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 75 kDa,77 kDa. We are unsure as to the identity of these extra bands.
  • Analysis of Human DDX46 by Immunoprecipitation in whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded) cells using ab72083 at 3 µg/mg lysate (lane 1) or a control rabbit IgG (lane 2). ab72083 was used for WB detection at 1 µg/ml. Detection: chemioluminescence, exposure time 3 seconds.

Anti-DDX46 antibody (ab72083)参考文献

ab72083 has not yet been referenced specifically in any publications.

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