Anti-DDX42抗体(ab89075)
Key features and details
- Mouse polyclonal to DDX42
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-DDX42抗体 -
描述
小鼠多克隆抗体to DDX42 -
宿主
Mouse -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human DDX42 aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q86XP3-2 -
阳性对照
- A431 cell lysate. Cell lysate from transfected 293T cells. HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab89075于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 10 µg/ml.
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 103 kDa.
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说明 |
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ICC/IF
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 103 kDa. |
靶标
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相关性
This gene encodes a member of the Asp-Glu-Ala-Asp (DEAD) box protein family. Members of this protein family are putative RNA helicases, and are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Two transcript variants encoding the same protein have been identified for this gene. -
细胞定位
Cytoplasm. Nucleus speckle. Nucleus> Cajal body. Note: Isoform 2 is present in Cajal bodies (CBs) and nuclear speckles. -
数据库链接
- Entrez Gene: 11325 Human
- SwissProt: Q86XP3 Human
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别名
- ATP-dependent RNA helicase DDX42 antibody
- DEAD (Asp-Glu-Ala-Asp) box polypeptide 42 antibody
- DEAD box polypeptide 42 protein antibody
see all
图片
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Anti-DDX42 antibody (ab89075) at 1 µg/ml + A431 cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 103 kDa
Observed band size: ~130 kDa why is the actual band size different from the predicted? -
All lanes : Anti-DDX42 antibody (ab89075) at 1 µg/ml
Lane 1 : cell lysate from DDX42 transfected 293T cells
Lane 2 : cell lysate from non transfected 293T cells
Lysates/proteins at 25 µg per lane.
Developed using the ECL technique.
Predicted band size: 103 kDa
Observed band size: ~130 kDa why is the actual band size different from the predicted? -
ab89075 at 10 µg/ml staining DDX42 in paraformaldehyde-fixed, permeabilised HeLa cells by immunofluorescence.
实验方案
数据表及文件
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Datasheet download
文献 (1)
ab89075 被引用在 1 文献中.
- Sun L et al. In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs. Cell 184:1865-1883.e20 (2021). PubMed: 33636127