Anti-DDX3Y抗体(ab95979)

概述

  • 产品名称Anti-DDX3Y抗体
    参阅全部 DDX3Y 一抗
  • 描述
    兔多克隆抗体to DDX3Y
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    Recombinant fragment, corresponding to a sequence within amino acids 70-316 of Human DDX3Y.

  • 阳性对照
    • 293T, A431, H1299, HeLa, HepG2, Molt4, and Raji cells and whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab95979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 73 kDa.
ICC/IF 1/100 - 1/200.

靶标

  • 功能Probable ATP-dependent RNA helicase. May play a role in spermatogenesis.
  • 组织特异性Testis-specific. Expressed predominantly in spermatogonia.
  • 疾病相关Note=DDX3Y is located in the 'azoospermia factor a' (AZFa) region on chromosome Y which is deleted in Sertoli cell-only syndrome. This is an infertility disorder in which no germ cells are visible in seminiferous tubules leading to azoospermia.
  • 序列相似性Belongs to the DEAD box helicase family. DDX3/DED1 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • 细胞定位Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm in an XPO1-dependent manner.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATP dependent RNA helicase DDX3Y antibody
    • ATP-dependent RNA helicase DDX3Y antibody
    • DBY antibody
    • DDX3Y antibody
    • DDX3Y_HUMAN antibody
    • DEAD (Asp-Glu-Ala-Asp) box polypeptide 3 Y linked antibody
    • DEAD box protein 3 Y chromosomal antibody
    • DEAD box protein 3 antibody
    • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide Y chromosome antibody
    • Y-chromosomal antibody
    see all

Anti-DDX3Y antibody 图像

  • All lanes : Anti-DDX3Y antibody (ab95979) at 1/10000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 73 kDa
  • ab95979 at 1/200 dilution staining DDX3Y in HeLa cells by Immunofluorescence, Paraformaldehyde-fixed. Lower image is merged with DNA probe.

Anti-DDX3Y antibody (ab95979)参考文献

ab95979 has not yet been referenced specifically in any publications.

Product Wall

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The lab has confirmed that the immunogen for ab95979 shares 96% identity with DDX3X, so it has high chance of cross-reacting with DDX3X which is why it is cross reacting with Hela cells.

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One possible reason of this cross reactivity is due to the sequence homology between DDX3Y and DDX3X. Please click the link below to see the DDX3Y sequence alignment with DDX3X; the similarity is 92% which is more ...

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I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future or...

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Thank you for contacting Abcam regarding ab95979.


I am sorry that the customer has experienced difficulties with this antibody in IF. I reviewed the protocol details provided and I would just like to clarify if the customer is using a h...

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