概述

  • 产品名称Anti-DDX3Y抗体
    参阅全部 DDX3Y 一抗
  • 描述
    小鼠多克隆抗体to DDX3Y
  • 特异性This antibody most likely cross-reacts with DDX3X, which shares 91% sequence homology with DDX3Y. This may be why ab72684 shows positive staining in HeLa cells, a female cell line, when DDX3Y is a Y-chromosome linked protein.
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length human DDX3Y protein (NP_004651.2)

  • 阳性对照
    • DDX3Y transfected 293T lysate and HeLa cells

性能

应用

Our Abpromise guarantee covers the use of ab72684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 73 kDa.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能Probable ATP-dependent RNA helicase. May play a role in spermatogenesis.
  • 组织特异性Testis-specific. Expressed predominantly in spermatogonia.
  • 疾病相关Note=DDX3Y is located in the 'azoospermia factor a' (AZFa) region on chromosome Y which is deleted in Sertoli cell-only syndrome. This is an infertility disorder in which no germ cells are visible in seminiferous tubules leading to azoospermia.
  • 序列相似性Belongs to the DEAD box helicase family. DDX3/DED1 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • 细胞定位Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm in an XPO1-dependent manner.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATP dependent RNA helicase DDX3Y antibody
    • ATP-dependent RNA helicase DDX3Y antibody
    • DBY antibody
    • DDX3Y antibody
    • DDX3Y_HUMAN antibody
    • DEAD (Asp-Glu-Ala-Asp) box polypeptide 3 Y linked antibody
    • DEAD box protein 3 Y chromosomal antibody
    • DEAD box protein 3 antibody
    • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide Y chromosome antibody
    • Y-chromosomal antibody
    see all

Anti-DDX3Y antibody 图像

  • All lanes : Anti-DDX3Y antibody (ab72684) at 1/500 dilution

    Lane 1 : DDX3Y transfected 293T lysate
    Lane 2 : Non transfected 293T lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 73 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • Purified ab72684, at 10µg/ml, staining DDX3Y in HeLa cells by Immunofluorescence.

Anti-DDX3Y antibody (ab72684)参考文献

ab72684 has not yet been referenced specifically in any publications.

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