概述

  • 产品名称Anti-DDX39抗体
    参阅全部 DDX39 一抗
  • 描述
    兔多克隆抗体to DDX39
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog, Zebrafish
  • 免疫原

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 53-395 of Human DDX39 (NP_005795)

  • 阳性对照
    • WB: 293T; A431; H1299; HeLaS3; HepG2; MOLT4 and Raji cell lysates ICC/IF: HeLa cells IHC-P: Gastric CA

性能

应用

Our Abpromise guarantee covers the use of ab96621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 49 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/200.

靶标

  • 相关性DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX39 encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates 2 transcript variants encoding different isoforms.
  • 细胞定位Cytoplasmic and Nuclear
  • 数据库链接
  • 别名
    • ATP dependent RNA helicase DDX39 antibody
    • ATP-dependent RNA helicase DDX39A antibody
    • BAT1 antibody
    • BAT1L antibody
    • DDX39 antibody
    • DDXL antibody
    • DEAD (Asp-Glu-Ala-Asp) box polypeptide 39 isoform 2 antibody
    • DEAD (Asp-Glu-Ala-Asp) box polypeptide 39A antibody
    • DEAD box protein 39 antibody
    • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 39 antibody
    • Eukaryotic initiation factor 4a antibody
    • Nuclear RNA helicase URH49 antibody
    • Nuclear RNA helicase, DECD variant of DEAD box family antibody
    • UAP56 related helicase antibody
    • UAP56 related helicase, 49 kDa antibody
    • URH49 antibody
    • wu:fc87b12 antibody
    • zgc:55881 antibody
    see all

Anti-DDX39 antibody 图像

  • Immunohistochemical analysis of DDX39 in paraffin embedded gastric CA, using ab96621 at a 1/100 dilution.
  • Immunofluorescence analysis of DDX39 in paraformaldehyde fixed HeLa, using ab96621 antibody at a 1/200 dilution: (1) without DNA probe, (2) merged with DNA probe.
  • All lanes : Anti-DDX39 antibody (ab96621) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : H1299 whole cell lysate
    Lane 4 : HeLaS3 whole cell lysate
    Lane 5 : HepG2 whole cell lysate
    Lane 6 : MOLT4 whole cell lysate
    Lane 7 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 49 kDa

Anti-DDX39 antibody (ab96621)参考文献

ab96621 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - nuclear (U2OS cells)
Gel Running Conditions Reduced Denaturing (13%)
Loading amount 15 µg
Specification U2OS cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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提交于 May 15 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"