Mouse, Rat, Cow, Dog, Zebrafish
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 53-395 of Human DDX39 (NP_005795)
WB: 293T; A431; H1299; HeLaS3; HepG2; MOLT4 and Raji cell lysates
ICC/IF: HeLa cells
IHC-P: Gastric CA
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 49 kDa.
1/100 - 1/250.
1/100 - 1/200.
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX39 encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates 2 transcript variants encoding different isoforms.
Cytoplasmic and Nuclear
ATP dependent RNA helicase DDX39 antibody
ATP-dependent RNA helicase DDX39A antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX39 antibody (ab96621)
Immunohistochemical analysis of DDX39 in paraffin embedded gastric CA, using ab96621 at a 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-DDX39 antibody (ab96621)
Immunofluorescence analysis of DDX39 in paraformaldehyde fixed HeLa, using ab96621 antibody at a 1/200 dilution: (1) without DNA probe, (2) merged with DNA probe.
Western blot - Anti-DDX39 antibody (ab96621)
All lanes : Anti-DDX39 antibody (ab96621) at 1/1000 dilution Lane 1 : 293T whole cell lysate Lane 2 : A431 whole cell lysate Lane 3 : H1299 whole cell lysate Lane 4 : HeLaS3 whole cell lysate Lane 5 : HepG2 whole cell lysate Lane 6 : MOLT4 whole cell lysate Lane 7 : Raji whole cell lysate Lysates/proteins at 30 µg per lane. Predicted band size: 49 kDa 10% SDS PAGE
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"