Anti-DDX39抗体[2252C4a] (ab50697)

概述

  • 产品名称Anti-DDX39抗体[2252C4a]
    参阅全部 DDX39 一抗
  • 描述
    小鼠单克隆抗体[2252C4a] to DDX39
  • 经测试应用适用于: WB, IP, Dot blotmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant fragment

  • 阳性对照
    • F2480 whole cell lysate. HeLa whole cell lysate. NIH3T3 whole cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab50697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
Dot blot
  • 应用说明Dot: Use at an assay dependent dilution.
    IP: Use at 5 µg.
    WB: 1/50 - 1/100. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX39 encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates 2 transcript variants encoding different isoforms.
    • 细胞定位Cytoplasmic and Nuclear
    • 数据库链接
    • 别名
      • ATP dependent RNA helicase DDX39 antibody
      • ATP-dependent RNA helicase DDX39A antibody
      • BAT1 antibody
      • BAT1L antibody
      • DDX39 antibody
      • DDXL antibody
      • DEAD (Asp-Glu-Ala-Asp) box polypeptide 39 isoform 2 antibody
      • DEAD (Asp-Glu-Ala-Asp) box polypeptide 39A antibody
      • DEAD box protein 39 antibody
      • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 39 antibody
      • Eukaryotic initiation factor 4a antibody
      • Nuclear RNA helicase URH49 antibody
      • Nuclear RNA helicase, DECD variant of DEAD box family antibody
      • UAP56 related helicase antibody
      • UAP56 related helicase, 49 kDa antibody
      • URH49 antibody
      • wu:fc87b12 antibody
      • zgc:55881 antibody
      see all

    Anti-DDX39 antibody [2252C4a] 图像

    • Anti-DDX39 antibody [2252C4a] (ab50697) + immunized recombinant protein

      Predicted band size : 49 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    • Anti-DDX39 antibody [2252C4a] (ab50697) at 1/100 dilution + HeLa whole cell lysate at 25 µg

      Secondary
      Mouse IgG antibody at 1/2500 dilution
      Developed using the ECL technique

      Predicted band size : 49 kDa
      Observed band size : 49 kDa
    • 500µg HeLa cell lysate was prepared in RIPA buffer and immunoprecipitated with 5µg ab50697. The subsequent Western blot was probed with ab50697 at 1/50, followed by TrueBlot anti-mouse IgG at 1/1000. Developed by the ECL technique.
    • Anti-DDX39 antibody [2252C4a] (ab50697) at 1/100 dilution + NIH3T3 whole cell lysate at 25 µg

      Secondary
      Mouse IgG antibody at 1/2500 dilution
      Developed using the ECL technique

      Predicted band size : 49 kDa
      Observed band size : 49 kDa
    • Anti-DDX39 antibody [2252C4a] (ab50697) at 1/100 dilution + F2480 whole cell lysate at 50 µg

      Secondary
      Mouse IgG antibody at 1/2500 dilution
      Developed using the ECL technique

      Predicted band size : 49 kDa
      Observed band size : 49 kDa

    Anti-DDX39 antibody [2252C4a] (ab50697)参考文献

    ab50697 has not yet been referenced specifically in any publications.

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