概述

  • 产品名称Anti-DDX31抗体
    参阅全部 DDX31 一抗
  • 描述
    兔多克隆抗体to DDX31
  • 经测试应用适用于: IP, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A region between residue 800 and the C-terminus (residue 851) of human DDX31 (NP_073616.6).

  • 阳性对照
    • HeLa and 293T whole cell lysates.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab70830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP
WB
  • 应用说明IP: Use at 2-5µg/mg of lysate.
    WB: 1/2500 - 1/10000. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性DDX31 is a member of DEAD box proteins family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The function of this DDX31 has not been determined.
    • 细胞定位Nucleus, nucleolus
    • 数据库链接
    • 别名
      • DEAD (Asp-Glu-Ala-Asp) box polypeptide 31 antibody
      • DEAD box protein 31 antibody
      • DEAD/DEXH helicase DDX31 antibody
      • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 31 antibody
      • FLJ13633 antibody
      • FLJ14578 antibody
      • FLJ23349 antibody
      • G2 helicase antibody
      • G2 Helicase B antibody
      • G2 Helicase C antibody
      • Gm997 antibody
      • Helicain antibody
      • Helicain B antibody
      • Helicain C antibody
      • Probable ATP dependent RNA helicase DDX31 antibody
      see all

    Anti-DDX31 antibody 图像

    • All lanes : Anti-DDX31 antibody (ab70830) at 0.4 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 95 kDa
      Observed band size : 95 kDa
      Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 3 minutes
    • Detection of DDX31 by Western Blot of Immunprecipitate.
      ab70830 at 1µg/ml staining DDX31 in HeLa whole cell lysate immunoprecipitated using ab70830 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 3 minutes.

    Anti-DDX31 antibody (ab70830)参考文献

    ab70830 has not yet been referenced specifically in any publications.

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