概述

  • 产品名称Anti-DDX3抗体
    参阅全部 DDX3 一抗
  • 描述
    兔多克隆抗体to DDX3
  • 特异性Detects both DDX3 and DBY
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to a 16 amino acid peptide from near the N terminus of Human DDX3

  • 阳性对照
    • HepG2 (Human hepatocellular liver carcinoma cell line) whole cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab37160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能ATP-dependent RNA helicase. Acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. Also involved in HIV-1 replication. Interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location.
  • 序列相似性Belongs to the DEAD box helicase family. DDX3/DED1 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • 细胞定位Nucleus speckle. Cytoplasm. Located predominantly in nuclear speckles and, at low levels, throughout the cytoplasm. Located to the outer side of nuclear pore complexes (NPC). Shuttles between the nucleus and the cytoplasm in a XPO1-dependent manner.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATP dependent RNA helicase DDX3X antibody
    • ATP-dependent RNA helicase DDX3X antibody
    • CAP Rf antibody
    • DBX antibody
    • DDX14 antibody
    • DDX3X antibody
    • DDX3X_HUMAN antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 3 X linked antibody
    • DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked antibody
    • DEAD box antibody
    • DEAD box protein 3 antibody
    • DEAD box protein 3 X-chromosomal antibody
    • DEAD box X isoform antibody
    • DEAD box, X isoform antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 3 antibody
    • DEAD/H box 3 antibody
    • DEAD/H box 3, X-linked antibody
    • Helicase like protein 2 antibody
    • Helicase-like protein 2 antibody
    • HLP2 antibody
    • X isoform antibody
    • X-chromosomal antibody
    see all

Anti-DDX3 antibody 图像

  • Lane 1 : Anti-DDX3 antibody (ab37160) at 0.5 µg/ml
    Lane 2 : Anti-DDX3 antibody (ab37160) at 1 µg/ml

    Lane 1 : Human HepG2 cell lysate
    Lane 2 : Human HepG2 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 73 kDa
    Observed band size : 73 kDa
  • Immunofluorescence of DDX3 in HepG2 cells using ab37160 at 20 ug/ml.

Anti-DDX3 antibody (ab37160)参考文献

This product has been referenced in:
  • King HA  et al. Remodelling of a polypyrimidine tract-binding protein complex during apoptosis activates cellular IRESs. Cell Death Differ N/A:N/A (2013). Read more (PubMed: 24141718) »
  • Jarboui MA  et al. Nucleolar Protein Trafficking in Response to HIV-1 Tat: Rewiring the Nucleolus. PLoS One 7:e48702 (2012). WB ; Human . Read more (PubMed: 23166591) »

See all 3 Publications for this product

Product Wall

Thank you for your enquiry. I am sorry to hear you are having a problem with ab37160. I would like to suggest the following modifications to your protocol: -Run a positive control of HepG2 (Human hepatocellular liver carcinoma cell line) whole ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"