纯化说明ab50703 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum and serile filtered.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Predicted molecular weight: 73 kDa.
Use at an assay dependent dilution.
功能ATP-dependent RNA helicase. Acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. Also involved in HIV-1 replication. Interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location.
序列相似性Belongs to the DEAD box helicase family. DDX3/DED1 subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
细胞定位Nucleus speckle. Cytoplasm. Located predominantly in nuclear speckles and, at low levels, throughout the cytoplasm. Located to the outer side of nuclear pore complexes (NPC). Shuttles between the nucleus and the cytoplasm in a XPO1-dependent manner.
DEAD (Asp Glu Ala Asp) box polypeptide 3 X linked antibody
DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked antibody
DEAD box antibody
DEAD box protein 3 antibody
DEAD box protein 3 X-chromosomal antibody
DEAD box X isoform antibody
DEAD box, X isoform antibody
DEAD/H (Asp Glu Ala Asp/His) box polypeptide 3 antibody
DEAD/H box 3 antibody
DEAD/H box 3, X-linked antibody
Helicase like protein 2 antibody
Helicase-like protein 2 antibody
X isoform antibody
Anti-DDX3 antibody [2253C5a] 图像
Western blot - DDX3 antibody [2253C5a] (ab50703)
Anti-DDX3 antibody [2253C5a] (ab50703) at 1/100 dilution + Hela whole cell lysate at 25 µg
Predicted band size : 73 kDa Observed band size : 73 kDa
Anti-DDX3 antibody [2253C5a] (ab50703)参考文献
This product has been referenced in:
Naji S et al. Host cell interactome of HIV-1 Rev includes RNA helicases involved in multiple facets of virus production. Mol Cell Proteomics11:M111.015313 (2012).
Read more (PubMed: 22174317) »