Anti-DDX28抗体(ab70821)
Key features and details
- Rabbit polyclonal to DDX28
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-DDX28抗体
参阅全部 DDX28 一抗 -
描述
兔多克隆抗体to DDX28 -
宿主
Rabbit -
经测试应用
适用于: IP, WBmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Gorilla -
免疫原
Synthetic peptide corresponding to a region between residue 500 and the C-terminus (residue 540) of human DDX28 (NP_060850.1).
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阳性对照
- HeLa and 293T whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70821于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
Use at 2 µg/mg of lysate.
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WB |
1/1000 - 1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
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说明 |
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IP
Use at 2 µg/mg of lysate. |
WB
1/1000 - 1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). |
靶标
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功能
May be involved in RNA processing or transport. Has RNA and Mg(2+)-dependent ATPase activity. -
组织特异性
Expressed in all tissues tested, including brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, leukocytes, colon, small intestine, ovary and prostate. -
序列相似性
Belongs to the DEAD box helicase family.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
细胞定位
Nucleus. Mitochondrion. Transported between these two compartments. Nuclear localization depends on active RNA polymerase II transcription. - Information by UniProt
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数据库链接
- Entrez Gene: 55794 Human
- Omim: 607618 Human
- SwissProt: Q9NUL7 Human
- Unigene: 458313 Human
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别名
- DDX 28 antibody
- DDX28 antibody
- DDX28_HUMAN antibody
see all
图片
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All lanes : Anti-DDX28 antibody (ab70821) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 60 kDa
Observed band size: 60 kDa
Additional bands at: 28 kDa. We are unsure as to the identity of these extra bands. -
Detection of Human DDX28 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70821 at 3 µg/mg lysate (Lane 1). Lane 2 represents rabbit IgG IP control. Subsequent Western blot detection of DDX28 was performed using ab70821 at 1 µg/ml.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab70821 被引用在 3 文献中.
- Evagelou SL et al. DEAD Box Protein Family Member DDX28 Is a Negative Regulator of Hypoxia-Inducible Factor 2a- and Eukaryotic Initiation Factor 4E2-Directed Hypoxic Translation. Mol Cell Biol 40:N/A (2020). PubMed: 31907278
- Reyes A et al. RCC1L (WBSCR16) isoforms coordinate mitochondrial ribosome assembly through their interaction with GTPases. PLoS Genet 16:e1008923 (2020). PubMed: 32735630
- Reyes A et al. RNase H1 Regulates Mitochondrial Transcription and Translation via the Degradation of 7S RNA. Front Genet 10:1393 (2019). PubMed: 32082360