Anti-DDX27抗体[2251C2a] (ab50696)

概述

  • 产品名称Anti-DDX27抗体[2251C2a]
    参阅全部 DDX27 一抗
  • 描述
    小鼠单克隆抗体[2251C2a] to DDX27
  • 经测试应用适用于: WB, Dot blot, ICCmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant human DDX27

  • 阳性对照
    • HeLa cells and HeLa and NIH3T3 cell lysates

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS (8mM Sodium phosphate, 3mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate), pH 7.4
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明ab50696 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22 µm membrane.
  • 克隆单克隆
  • 克隆编号2251C2a
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab50696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50. Predicted molecular weight: 90 kDa.
Dot blot Use at an assay dependent dilution.
ICC 1/5.

靶标

  • 功能Probable ATP-dependent RNA helicase.
  • 序列相似性Belongs to the DEAD box helicase family. DDX27/DRS1 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C86129 antibody
    • cb843 antibody
    • DDX27 antibody
    • DDX27_HUMAN antibody
    • DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 antibody
    • DEAD box protein 27 antibody
    • Deficiency of ribosomal subunits protein 1 homolog antibody
    • dJ686N3.1 antibody
    • DKFZp667N057 antibody
    • DRS1 antibody
    • FLJ12917 antibody
    • FLJ20596 antibody
    • FLJ22238 antibody
    • HSPC259 antibody
    • MGC1018 antibody
    • MGC163147 antibody
    • PP3241 antibody
    • Probable ATP-dependent RNA helicase DDX27 antibody
    • RHLP antibody
    • RNA helicase like protein antibody
    • Rrp3p antibody
    see all

Anti-DDX27 antibody [2251C2a] 图像

  • Anti-DDX27 antibody [2251C2a] (ab50696) at 1/50 dilution + HeLa whole cell lysate at 25 µg

    Secondary
    Mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 90 kDa
    Observed band size : 90 kDa
  • Anti-DDX27 antibody [2251C2a] (ab50696) at 1/50 dilution + NIH3T3 whole cell lyaste at 25 µg

    Secondary
    Mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 90 kDa
    Observed band size : 90 kDa
  • ab50696 at 1/5 dilution staining human DDX27 in HeLa cells by Immunocytochemistry. A 1/200 dilution of AlexaFluor488 Goat Anti-mouse IgG was used as secondary.

Anti-DDX27 antibody [2251C2a] (ab50696)参考文献

ab50696 has not yet been referenced specifically in any publications.

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