概述

  • 产品名称Anti-DDX24抗体
    参阅全部 DDX24 一抗
  • 描述
    兔多克隆抗体to DDX24
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 325 and 375 of Human DDX24 (NP_065147.1)

  • 阳性对照
    • HeLa, 293T and NIH3T3 whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab70463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能ATP-dependent RNA helicase.
    • 组织特异性Ubiquitous. Most abundant in heart and brain, but with lowest levels in thymus and small intestine.
    • 序列相似性Belongs to the DEAD box helicase family. DDX24/MAK5 subfamily.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
    • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ATP dependent RNA helicase DDX24 antibody
      • ATP-dependent RNA helicase ddx24 antibody
      • DDX 24 antibody
      • ddx24 antibody
      • DDX24_HUMAN antibody
      • DEAD (Asp Glu Ala Asp) box polypeptide 24 antibody
      • DEAD box protein 24 antibody
      • DEAD/H (Asp Glu Ala Asp) box polypeptide 24 antibody
      • S. cerevisiae CHL1 like helicase antibody
      see all

    Anti-DDX24 antibody 图像

    • All lanes : Anti-DDX24 antibody (ab70463) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : NIH3T3 whole cell lysate at 50 µg


      Predicted band size : 97 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 160 kDa,230 kDa,350 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 10 seconds

    Anti-DDX24 antibody (ab70463)参考文献

    ab70463 has not yet been referenced specifically in any publications.

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