Anti-DDX24抗体(ab70463)
Key features and details
- Rabbit polyclonal to DDX24
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-DDX24抗体
参阅全部 DDX24 一抗 -
描述
兔多克隆抗体to DDX24 -
宿主
Rabbit -
经测试应用
适用于: IP, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to a region between residue 325 and 375 of Human DDX24 (NP_065147.1)
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阳性对照
- WB: HeLa, HEK293T, NIH/3T3 whole cell lysates. IP: HeLa
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Antibody was affinity purified using an epitope specific to DDX24 immobilized on solid support -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70463于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
Use at 2-5 µg/mg of lysate.
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WB |
1/1000 - 1/5000. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa).
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说明 |
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IP
Use at 2-5 µg/mg of lysate. |
WB
1/1000 - 1/5000. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa). |
靶标
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功能
ATP-dependent RNA helicase. -
组织特异性
Ubiquitous. Most abundant in heart and brain, but with lowest levels in thymus and small intestine. -
序列相似性
Belongs to the DEAD box helicase family. DDX24/MAK5 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. - Information by UniProt
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数据库链接
- Entrez Gene: 57062 Human
- Entrez Gene: 27225 Mouse
- Omim: 606181 Human
- SwissProt: Q9GZR7 Human
- SwissProt: Q9ESV0 Mouse
- SwissProt: Q5RDL2 Orangutan
- Unigene: 510328 Human
- Unigene: 3935 Mouse
see all -
别名
- ATP dependent RNA helicase DDX24 antibody
- ATP-dependent RNA helicase ddx24 antibody
- DDX 24 antibody
see all
图片
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DDX24 was immunoprecipitated from HeLa whole cell lysate (1 mg per IP reaction, 20% loaded) with ab70463 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab70463 at 1 µg/mL.
Lane 1: ab70463 IP in HeLa whole cell lysate.
Lane 2: ab70463 IP in HeLa whole cell lysate.
Lane 3: Control IgG in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 minutes
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All lanes : Anti-DDX24 antibody (ab70463) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HEK293T whole cell lysate
Lane 3 : NIH/3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 97 kDa
Exposure time: 3 minutes
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab70463 被引用在 1 文献中.
- Wu T et al. Characterization of R-Loop-Interacting Proteins in Embryonic Stem Cells Reveals Roles in rRNA Processing and Gene Expression. Mol Cell Proteomics 20:100142 (2021). PubMed: 34478875