概述

  • 产品名称
  • 描述
    小鼠多克隆抗体to DDX24
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length human DDX24 protein (AAH96826.1, 804 a.a).

  • 阳性对照
    • human liver lysate, DDX24 transfected 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab69148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 100, 150 kDa (predicted molecular weight: 95 kDa).

靶标

  • 功能
    ATP-dependent RNA helicase.
  • 组织特异性
    Ubiquitous. Most abundant in heart and brain, but with lowest levels in thymus and small intestine.
  • 序列相似性
    Belongs to the DEAD box helicase family. DDX24/MAK5 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATP dependent RNA helicase DDX24 antibody
    • ATP-dependent RNA helicase ddx24 antibody
    • DDX 24 antibody
    • ddx24 antibody
    • DDX24_HUMAN antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 24 antibody
    • DEAD box protein 24 antibody
    • DEAD/H (Asp Glu Ala Asp) box polypeptide 24 antibody
    • S. cerevisiae CHL1 like helicase antibody
    see all

Anti-DDX24 antibody 图像

  • Anti-DDX24 antibody (ab69148) at 1/500 dilution + human liver lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 95 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-DDX24 antibody (ab69148) at 1/500 dilution

    Lane 1 : DDX24 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 95 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)

实验方案

Anti-DDX24 antibody (ab69148)参考文献

ab69148 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab69148.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册