概述

  • 产品名称Anti-DDX24抗体
    参阅全部 DDX24 一抗
  • 描述
    小鼠多克隆抗体to DDX24
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length human DDX24 protein (AAH96826.1, 804 a.a).

  • 阳性对照
    • human liver lysate, DDX24 transfected 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab69148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 100, 150 kDa (predicted molecular weight: 95 kDa).

靶标

  • 功能ATP-dependent RNA helicase.
  • 组织特异性Ubiquitous. Most abundant in heart and brain, but with lowest levels in thymus and small intestine.
  • 序列相似性Belongs to the DEAD box helicase family. DDX24/MAK5 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATP dependent RNA helicase DDX24 antibody
    • ATP-dependent RNA helicase ddx24 antibody
    • DDX 24 antibody
    • ddx24 antibody
    • DDX24_HUMAN antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 24 antibody
    • DEAD box protein 24 antibody
    • DEAD/H (Asp Glu Ala Asp) box polypeptide 24 antibody
    • S. cerevisiae CHL1 like helicase antibody
    see all

Anti-DDX24 antibody 图像

  • Anti-DDX24 antibody (ab69148) at 1/500 dilution + human liver lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 95 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-DDX24 antibody (ab69148) at 1/500 dilution

    Lane 1 : DDX24 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 95 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)

Anti-DDX24 antibody (ab69148)参考文献

ab69148 has not yet been referenced specifically in any publications.

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