Anti-DDX23抗体(ab70461)

概述

  • 产品名称Anti-DDX23抗体
    参阅全部 DDX23 一抗
  • 描述
    兔多克隆抗体to DDX23
  • 经测试应用适用于: ICC/IF, WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • 免疫原

    Synthetic peptide corresponding to a region between residue 775 and the C-terminus (residue 820) of Human DDX23 (NP_004809.2)

  • 阳性对照
    • Whole cell lysate from HeLa, 293T and NIH3T3 cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab70461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/500.
WB 1/2000 - 1/10000. Detects a band of approximately 96 kDa (predicted molecular weight: 96 kDa).
IP Use at 2 µg/mg of lysate.

靶标

  • 功能Involved in pre-mRNA splicing and its phosphorylated form (by SRPK2) is required for spliceosomal B complex formation.
  • 序列相似性Belongs to the DEAD box helicase family. DDX23/PRP28 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • 翻译后修饰In vitro phosphorylated by CLK1 and U1 snRNP-associated protein kinase. Phosphorylated by SRPK2 and this phosphorylation is required for its association with the tri-snRNP (U4/U6-U5 tri-small nuclear ribonucleoproteins) and subsequent spliceosomal B complex formation.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 100 kDa U5 snRNP-specific protein antibody
    • DDX23 antibody
    • DDX23_HUMAN antibody
    • DEAD box protein 23 antibody
    • EC 3.6.1.- antibody
    • Probable ATP-dependent RNA helicase DDX23 antibody
    • PRP28 homolog antibody
    • U5-100kD antibody
    see all

Anti-DDX23 antibody 图像

  • All lanes : Anti-DDX23 antibody (ab70461) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 96 kDa
    Observed band size : 96 kDa
    Additional bands at : 200 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • ab70461 staining DDX23 in murine NIH/3T3 cells by ICC/IF (Immunocytochemistry/immunofluorescence).

    Cells were fixed with formaldehyde and blocked with 5% serum for 20 minutes at 21°C. Samples were incubated with primary antibody (1/50 in 5% goat serum) for 16 hours at 4°C. An AlexaFluor®594-conjugated goat anti-rabbit polyclonal (1/200) was used as the secondary antibody. Nuclei were stained with DAPI.

    See Abreview

Anti-DDX23 antibody (ab70461)参考文献

ab70461 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (NIH/3T3)
Specification NIH/3T3
Fixative Formaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mrs. Filomena Ricciardi

Verified customer

提交于 Jul 02 2012



DISCOUNT CODE: 00000


Ablaufdatum: 05. Oktober 2012

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