The immunogen sequence is highly conserved with DDX19A (GeneID 55308), but crossreactivity with this homolog has not been assessed.
Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
Synthetic peptide corresponding to a region between residues 400 and 450 of human DDX19B. (NP_009173.1)
HeLa, 293T and NIH3T3 whole cell lystes.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).
Use at 2 µg/mg of lysate.
ATP-dependent RNA helicase involved in mRNA export from the nucleus.
Belongs to the DEAD box helicase family. DDX19/DBP5 subfamily.
Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Cytoplasm. Nucleus > nuclear pore complex. Nucleus membrane. Nuclear pore complex cytoplasmic fibrils.
Information by UniProt
ATP-dependent RNA helicase DDX19B antibody
Dbp5, yeast, homolog of antibody
Western blot - DDX19B antibody (ab70306)
All lanes :
Anti-DDX19B antibody (ab70306) at 0.04 µg/ml
Lane 1 :
HeLa whole cell lysate at 50 µg
Lane 2 :
HeLa whole cell lysate at 15 µg
Lane 3 :
HeLa whole cell lysate at 5 µg
Lane 4 :
293T whole cell lysate at 50 µg
Lane 5 :
NIH3T3 whole cell lysate at 50 µg
Predicted band size :
Observed band size :
60 kDa (
why is the actual band size different from the predicted?
Exposure time :
has not yet been referenced specifically in any publications.
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