The immunogen sequence is highly conserved with respect to DDX19A (GeneID 55308), but crossreactivity of the antibody with this homolog has not been assessed.
Rat, Guinea pig, Cow, Xenopus laevis, Zebrafish, Orangutan, Xenopus tropicalis, Other
Synthetic peptide corresponding to a region between C terminal residues 450 and 479 of Human DDX19B. (NP_009173.1)
Whole cell lysate from HeLa , 293T and NIH3T3 cells
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
Use at 2-5 µg/mg of lysate.
1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ATP-dependent RNA helicase involved in mRNA export from the nucleus.
Belongs to the DEAD box helicase family. DDX19/DBP5 subfamily.
Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Cytoplasm. Nucleus > nuclear pore complex. Nucleus membrane. Nuclear pore complex cytoplasmic fibrils.
Information by UniProt
ATP-dependent RNA helicase DDX19B antibody
Dbp5, yeast, homolog of antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX19B antibody (ab70207)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling DDX19B with ab70207 at 1/200 (1µg/ml). Detection: DAB.
Western blot - DDX19B antibody (ab70207)
All lanes : Anti-DDX19B antibody (ab70207) at 0.04 µg/ml Lane 1 : Whole cell lysate from HeLa cells at 50 µg Lane 2 : Whole cell lysate from HeLa cells at 15 µg Lane 3 : Whole cell lysate from HeLa cells at 5 µg Lane 4 : Whole cell lysate from 293T cells at 50 µg Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg Predicted band size : 53 kDa Observed band size : 53 kDa Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands. Exposure time : 10 seconds
has not yet been referenced specifically in any publications.
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